Assessment of Cell-Free DNA with Microvascular Complication of Type II Diabetes Mellitus, Using PCR and Elisa

• Published in: Nucleosides, nucleotides & nucleic acids Vol. 29; no. 3; pp. 228 - 236
• Main Authors: El Tarhouny, Shereen A., Hadhoud, Khaled M., Ebrahem, Magdy M., Al Azizi, Nashwa M.
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis Group 01-03-2010
• Subjects: Aged; cell-free DNA; Cells; Diabetes Complications - blood; Diabetes Complications - genetics; Diabetes Mellitus, Type 2 - blood; Diabetes Mellitus, Type 2 - genetics; DNA - blood; DNA - genetics; Enzyme-Linked Immunosorbent Assay; Female; Humans; Male; microvascular complications; Middle Aged; Nucleosomes; Nucleosomes - chemistry; Reverse Transcriptase Polymerase Chain Reaction; Sensitivity and Specificity; type 2 diabetes mellitus
• ISSN: 1525-7770; 1532-2335
• DOI: 10.1080/15257771003708298

In order to assess the potential biochemical markers in the development, diagnosis, and prognosis of diabetic patient with microvascular complication represented with retinopathy, we analyzed the levels of cell-free DNA by two different techniques. The levels of cell-free GAPDH assayed by quantitative PCR were significantly higher in the plasma samples of diabetic patients with and without diabetic retinopathy than in those of the control group; thus, it is a better biomarker than nucleosomes assayed by ELISA in patients with type 2 diabetes for the early detection of development of microvasuclar complications as retinopathy.