Starch metabolism in germinating soybean cotyledons is sensitive to clinorotation and centrifugation

• Published in: Plant, cell and environment Vol. 17; p. 341
• Main Authors: Brown, C S, Piastuch, W C
• Format: Journal Article
• Language: English
• Published: United States 01-03-1994
• Subjects: Centrifugation; Chromatography, High Pressure Liquid; Cotyledon - chemistry; Cotyledon - enzymology; Cotyledon - metabolism; Germination - physiology; Glucose-1-Phosphate Adenylyltransferase; Glycine max - chemistry; Glycine max - enzymology; Glycine max - metabolism; Gravitation; Gravity, Altered; Lipid Metabolism; Lipids - analysis; Nucleotidyltransferases - analysis; Nucleotidyltransferases - metabolism; Rotation; Starch - analysis; Starch - metabolism; Time Factors; Weightlessness Simulation; NASA Center KSC; NASA Discipline Number 40-50; NASA Discipline Plant Biology
• DOI: 10.1111/j.1365-3040.1994.tb00301.x
• ISSN: 0140-7791

Soybean (Glycine max [L.] Merr. cv. McCall) seedlings germinated and grew for 6d under the altered gravity conditions of horizontal clinorotation and centrifugation. Both of these conditions resulted in decreased growth relative to the control (vertically rotated) plants. Starch concentration in the cotyledons was lower in the clinorotated plants and was higher in the centrifuged plants compared to the controls. The opposite relationship was noted for total lipid concentration. Of the six starch metabolic enzyme activities measured, only ADP glucose pyrophosphorylase was affected by the gravity treatments; being lower in the cotyledons of the horizontally rotated plants and higher in the cotyledons of the centrifuged plants relative to the control values.