Efficient production of 2,3-butanediol from corn stover hydrolysate by using a thermophilic Bacillus licheniformis strain

• Published in: Bioresource technology Vol. 170; pp. 256 - 261
• Main Authors: Li, Lixiang, Li, Kun, Wang, Kai, Chen, Chao, Gao, Chao, Ma, Cuiqing, Xu, Ping
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01-10-2014; Elsevier
• Subjects: 2,3-Butanediol; Agronomy. Soil science and plant productions; Bacillus - genetics; Bacillus - metabolism; Bacillus licheniformis; Biological and medical sciences; Butylene Glycols - metabolism; Carbon catabolite repression; Chromatography, Gas; Chromatography, High Pressure Liquid; Corn stover hydrolysate; DNA Primers - genetics; Fermentation; Food industries; Fundamental and applied biological sciences. Psychology; General agronomy. Plant production; Glucose - metabolism; Industrial Microbiology - methods; Lignin - metabolism; RNA, Ribosomal, 16S - genetics; Species Specificity; Spectrophotometry; Use and upgrading of agricultural and food by-products. Biotechnology; Use of agricultural and forest wastes. Biomass use, bioconversion; Xylose - metabolism; Zea mays - chemistry; Carbon catabolite repression; Corn stover hydrolysate; Bacillus licheniformis; 2,3-Butanediol; Monocotyledones; Zea mays; Alkanediol; Thermophily; Repression; Bacillaceae; Carbon; Cereal crop; Straw; Catabolite; Bacillales; Hydrolysate; Gramineae; Angiospermae; Bacteria; Spermatophyta; Corn by product
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2014.07.101

•Thermophilic Bacillus licheniformis X10 utilizes glucose and xylose simultaneously.•B. licheniformis X10 tolerates up to 3g/L of furfural, vanillin, or formic acid.•A high production of 2,3-BD (74.0g/L) was achieved from corn stover hydrolysate.
In this study, a thermophilic Bacillus licheniformis strain X10 was newly isolated for 2,3-butanediol (2,3-BD) production from lignocellulosic hydrolysate. Strain X10 could utilize glucose and xylose simultaneously without carbon catabolite repression. In addition, strain X10 possesses high tolerance to fermentation inhibitors including furfural, vanillin, formic acid, and acetic acid. In a fed-batch fermentation, 74.0g/L of 2,3-BD was obtained from corn stover hydrolysate, with a productivity of 2.1g/Lh and a yield of 94.6%. Thus, this thermophilic B. licheniformis strain is a candidate for the development of efficient industrial production of 2,3-BD from corn stover hydrolysate.