Effect of monomer blend and chlorhexidine-adding on physical, mechanical and biological properties of experimental infiltrants

• Published in: Dental materials Vol. 32; no. 12; pp. e307 - e313
• Main Authors: Inagaki, Luciana Tiemi, Alonso, Roberta Caroline Bruschi, Araújo, Giovana Albamonte Spagnolo, de Souza-Junior, Eduardo José Carvalho, Anibal, Paula Cristina, Höfling, José Francisco, Pascon, Fernanda Miori, Puppin-Rontani, Regina Maria
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-12-2016
• Subjects: Advanced Basic Science; Analysis of variance; Antiinfectives and antibacterials; Antimicrobial activity; BISEMA; Blends; Chlorhexidine; Composite Resins; Concentration (composition); Degree of conversion; Dentistry; Hardness; Inhibition; Knoop hardness; Lactobacillus acidophilus; Materials Testing; Monomers; Polymer blends; Polymerization; Streptococcus mutans; TEGDMA; UDMA; Degree of conversion; Antimicrobial activity; UDMA; TEGDMA; BISEMA; Chlorhexidine; Knoop hardness
• ISSN: 0109-5641; 1879-0097
• DOI: 10.1016/j.dental.2016.09.028

Abstract Objectives The aim of the study was to evaluate antimicrobial inhibition zone, degree of conversion (DC) and Knoop hardness (KH) of experimental infiltrants. Experimental low viscosity monomer blends were prepared and chlorhexidine diacetate salt (CHX) (0.1% or 0.2%) was added comprising the groups: G1) TEGDMA; G2) TEGDMA/0.1CHX; G3) TEGDMA/0.2CHX; G4) TEGDMA/UDMA; G5) TEGDMA/UDMA/0.1CHX; G6) TEGDMA/UDMA/0.2CHX; G7) TEGDMA/BISEMA; G8) TEGDMA/BISEMA/0.1CHX; G9) TEGDMA/BISEMA/0.2CHX. Icon® was used as control group. Methods Specimens of resin blends were made ( n = 9) to accomplished DC and KH. Pour plate was accomplished to evaluate antimicrobial groups’ activity against Streptococcus mutans (SM) and Lactobacillus acidophilus (LA). Data obtained were submitted to two-way ANOVA and Tukey tests for blends comparisons and Dunnett's test for comparisons between experimental infiltrants and Icon® ( p < 0.05). Results In relation to antimicrobial effect, uncured blends showed higher antibacterial activity than cured ones for the most of blends. After polymerization, G5 showed the highest inhibition zone against SM and, G3 and G6 against LA. Concerning KH, TEGDMA/UDMA-based blends showed the highest values of KH number and it was influenced by monomeric base, regardless CHX concentration. DC was not affected by monomer blend composition, neither for CHX concentration. The antimicrobial activity was affected by monomeric base, CHX concentration and polymerization. Experimental infiltrants presented similar or higher performance than Icon® for the properties evaluated. Significance White spot lesion infiltration with low viscosity monomer blends (infiltrants) is an alternative to stop initial caries lesions progression. The incorporation of an antimicrobial agent as chlorhexidine diacetate salt in infiltrants composition could enhance the performance of these materials.