Monoclonal antibodies for the detection and quantitation of the endogenous plant growth regulator, abscisic acid

• Published in: FEBS letters Vol. 160; no. 1; pp. 269 - 272
• Main Authors: Mertens, R., Deus-Neumann, B., Weiler, E.W.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 22-08-1983; Elsevier
• Subjects: ABA, abscisic acid; ABAG, ABA-β-D-glucopyranosyl ester; Abscisic acid; Analytical, structural and metabolic biochemistry; Biological and medical sciences; DP, dihydrophaseic acid; FCS; fetal calf serum; Fundamental and applied biological sciences. Psychology; mAB, monoclonal antibodies; Monoclonal antibody; Monoclonal antibody radioimmunoassay; Other biological molecules; PA, phaseic acid, pAB, polyclonal antibodies; Plant growth regulator; Plant growth substances; RIA, radioimmunoassay, TLC, thin layer chromatography; ABAG, ABA-β-D-glucopyranosyl ester; PA, phaseic acid, pAB, polyclonal antibodies; Monoclonal antibody radioimmunoassay; DP, dihydrophaseic acid; RIA, radioimmunoassay, TLC, thin layer chromatography; FCS; Abscisic acid; fetal calf serum; Monoclonal antibody; Plant growth regulator; ABA, abscisic acid; mAB, monoclonal antibodies; Assay; Stereoisomer; Plant growth substance; Preparation
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(83)80980-6

Monoclonal antibodies (mAB) have been produced which recognize the physiologically active 2-
cis-(
S-form of the endogenous plant growth regulator, abscisic acid (ABA). Cross-reaction with the ABA-catabolites, phaseic and dihydrophaseic acid, is negligible, and (
R)-ABA, 2-
trans-ABA, the ABA-conjugate, ABA-β-D-glucopyranosyl ester, as well as the putative ABA precursor, xanthoxin, are totally unreactive. In addition to being very specific, the mAB exhibit high affinites for 2-
cis-(
S)-ABA: the
K values were 7.9 × 10
9 l/mol and 3.7 × 10
9 l/mol for antibodies from two different clones. By mAB-radioimmunoassay (RIA), 4 pg 2-
cis-(
S)-ABA (99.5% confidence level) can be detected. mAB-RIA can be used to quantitate ABA directly in unprocessed plant extracts.