Detection of leptospires in bovine semen by polymerase chain reaction
Objective In view of the considerable importance of vene real transmission of bovine leptospirosis, the objective of the present study was to compare the polymerase chain reaction (PCR), culture/isolation and serology to detect leptospire infection in bovine semen. Design Blood for serologic examina...
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| Published in: | Australian veterinary journal Vol. 77; no. 1; pp. 32 - 34 |
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| Main Authors: | , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
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Oxford, UK
Blackwell Publishing Ltd
1999
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| Abstract | Objective In view of the considerable importance of vene real transmission of bovine leptospirosis, the objective of the present study was to compare the polymerase chain reaction (PCR), culture/isolation and serology to detect leptospire infection in bovine semen.
Design Blood for serologic examination and semen for bacterial culture and PCR were collected from 20 bulls at arti ficial insemination centres in Brazil. Each animal was sampled twice for serology.
Result Forty‐five percent (9/20) of the serum samples collected showed agglutinin titers to serovar hardjo i n the first sample and 25% (5/20) had agglutinin titers to serovar hardjo in the second sample. Eighty percent (16/20) of semen samples were positive by PCR. Leptospires could not be isolated from any of the semen samples examined.
Conclusion Polymerase chain reaction can be a method of great potential for the detection of leptospires at artificial insemination centres. |
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| AbstractList | Objective In view of the considerable importance of vene real transmission of bovine leptospirosis, the objective of the present study was to compare the polymerase chain reaction (PCR), culture/isolation and serology to detect leptospire infection in bovine semen. Design Blood for serologic examination and semen for bacterial culture and PCR were collected from 20 bulls at arti ficial insemination centres in Brazil. Each animal was sampled twice for serology. Result Forty-five percent (9/20) of the serum samples collected showed agglutinin titers to serovar hardjo i n the first sample and 25% (5/20) had agglutinin titers to serovar hardjo in the second sample. Eighty percent (16/20) of semen samples were positive by PCR. Leptospires could not be isolated from any of the semen samples examined. Conclusion Polymerase chain reaction can be a method of great potential for the detection of leptospires at artificial insemination centres. Objective In view of the considerable importance of vene real transmission of bovine leptospirosis, the objective of the present study was to compare the polymerase chain reaction (PCR), culture/isolation and serology to detect leptospire infection in bovine semen. Design Blood for serologic examination and semen for bacterial culture and PCR were collected from 20 bulls at arti ficial insemination centres in Brazil. Each animal was sampled twice for serology. Result Forty‐five percent (9/20) of the serum samples collected showed agglutinin titers to serovar hardjo i n the first sample and 25% (5/20) had agglutinin titers to serovar hardjo in the second sample. Eighty percent (16/20) of semen samples were positive by PCR. Leptospires could not be isolated from any of the semen samples examined. Conclusion Polymerase chain reaction can be a method of great potential for the detection of leptospires at artificial insemination centres. In view of the considerable importance of venereal transmission of bovine leptospirosis, the objective of the present study was to compare the polymerase chain reaction (PCR), culture/isolation and serology to detect leptospire infection in bovine semen. Blood for serologic examination and semen for bacterial culture and PCR were collected from 20 bulls at artificial insemination centres in Brazil. Each animal was sampled twice for serology. Forty-five percent (9/20) of the serum samples collected showed agglutinin titers to serovar hardjo in the first sample and 25% (5/20) had agglutinin titers to serovar hardjo in the second sample. Eighty percent (16/20) of semen samples were positive by PCR. Leptospires could not be isolated from any of the semen samples examined. Polymerase chain reaction can be a method of great potential for the detection of leptospires at artificial insemination centres. Objective In view of the considerable importance of vene real transmission of bovine leptospirosis, the objective of the present study was to compare the polymerase chain reaction (PCR), culture/isolation and serology to detect leptospire infection in bovine semen. Design Blood for serologic examination and semen for bacterial culture and PCR were collected from 20 bulls at arti ficial insemination centres in Brazil. Each animal was sampled twice for serology. Result Forty‐five percent (9/20) of the serum samples collected showed agglutinin titers to serovar hardjo i n the first sample and 25% (5/20) had agglutinin titers to serovar hardjo in the second sample. Eighty percent (16/20) of semen samples were positive by PCR. Leptospires could not be isolated from any of the semen samples examined. Conclusion Polymerase chain reaction can be a method of great potential for the detection of leptospires at artificial insemination centres. OBJECTIVEIn view of the considerable importance of venereal transmission of bovine leptospirosis, the objective of the present study was to compare the polymerase chain reaction (PCR), culture/isolation and serology to detect leptospire infection in bovine semen.DESIGNBlood for serologic examination and semen for bacterial culture and PCR were collected from 20 bulls at artificial insemination centres in Brazil. Each animal was sampled twice for serology.RESULTForty-five percent (9/20) of the serum samples collected showed agglutinin titers to serovar hardjo in the first sample and 25% (5/20) had agglutinin titers to serovar hardjo in the second sample. Eighty percent (16/20) of semen samples were positive by PCR. Leptospires could not be isolated from any of the semen samples examined.CONCLUSIONPolymerase chain reaction can be a method of great potential for the detection of leptospires at artificial insemination centres. |
| Author | MORAIS, ZM GARCIA, JF GREGORI, F. RICHTZENHAIN, LJ NUNES, CM HEINEMANN, MB CORTEZ, A. VASCONCELLOS, SA VISINTIN, JA |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/10028391$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1128/jcm.27.10.2258-2262.1989 10.1136/vr.118.12.333 10.1136/vr.108.26.555 10.1080/00480169.1980.34741 10.1016/S0034-5288(18)31717-X 10.11606/issn.2318-3659.v25i2p221-235 10.1136/vr.119.4.84 10.1128/jcm.30.9.2219-2224.1992 10.1136/vr.110.8.178 10.1136/vr.118.1.11 |
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| References | Passos EC , Vasconcellos SA , Ito FH , Yasuda PH , Nürmberger Junior R. . Isolamento de leptospiras a partir do tecido renal de hamsters experimental-mente infectados com Leptospira interrogans serotipo pomona. Emprego das técnicas da pipeta Pasteur e das diluições seriadas em meios de cultura de Fletcher tratado com 5-fluor-uracil ou o sulfato de neomicina . Rev Fac Med Vet Zootec USP 1988 ; 25 : 221 - 235 . Ellis WA , O'Brien JJ , Neill SD , Hanna J. . Bovine leptospirosis: serological findings in aborting cows . Vet Rec 1982 ; 20 : 178 - 180 . Thiermann AB . Bovine leptospirosis: bacteriologic versus serologic diagnosis of cows at slaughter . Am J Vet Res 1983 ; 44 : 2244 - 2245 . Ellis WA , O'Brien JJ , Cassells JA , Neill SD , Hanna J. Excretion of Leptospira interrogans serovar hardjo following calving or abortion . Res Vet Sci 1985 ; 39 : 296 - 98 . Bolin CA , Zuerner RL , Trueba G. Comparison of three techniques to detect Leptospira interrogans serovar hardjo type hardjo-bovis in bovine urine . Am J Vet Res 1989 ; 50 : 1001 - 1003 . Guimarães MC, Santa Rosa CA, Vasconcellos SA, Oliveira JEAS, Cõrtes JA. Diagnóstico da leptospirose em bovinos doadores de sêmen. Pesquisa de aglutininas séricas e tentativa de isolamento do agente a partir de amostras de sêmen . Rev Bras Reprod Anim 1987 ; 31 : 136 - 140 . Van Eys GJJM , Gravekamp C. , Gerritsen MJ et al. Detection of leptospires in urine by polymerase chain reaction . J Clin Microbiol 1989 ; 27 : 2258 - 2262 . Guimarães MC, Côrtes JA, Vasconcellos SA, Ito FH. Epidemiologia e controle da leptospirose em bovinos. Papel do portador e seu controle terapêutico . Comun Cient Fac Med Vet Zootec USP 1982 ; 1/4 : 21 - 25 . Eaglesome MD , Garcia MM. Microbial agents associated with bovine genital tract infections and semen. Part I. Brucella abortus, Leptospira, Campylobacter fetus and Trichomonas foetus . Vet Bul 1992 ; 62 : 743 - 75 . Ellis WA , Songer JG , Montgomery J. , Cassells , JA . Prevalence of Leptospira interrogans serovar hardjo in the genital tracts of non-pregnant cattle . Vet Rec 1986 ; 118 : 11 - 13 . Hathaway SC , Little TWA , Pritchard DG . Problems associated with the serological diagnosis of Leptospira interrogans serovar hardjo infection in bovine populations . Vet Rec 1986 ; 119 : 84 - 86 . Schönberg A. , Kampe U. , Rohloff D. Methods for the detection of leptospires in sperm samples of boar . Berl Munch Tierarztl Wochenschr 1980 ; 93 : 166 - 171 . Mérien F. , Amouriax P. , Perolat P. , Baranton G. , Saint Girons I. Polymerase chain reaction for detection of Leptospira spp. in clinical samples . J Clin Microbiol 1992 ; 30 : 2219 - 2224 . Mackintosh CG , Marshall RB , Broughton ES . The use of a hardjo-pomona vaccine to prevent leptospiruria in cattle exposed to natural challenge with Leptospira interrogans serovar hardjo . N Z Vet J 1980 ; 28 : 174 - 177 . Sleight SD , William's JA . Transmission of bovine leptospirosis by coition and artificial insemination - A preliminary report . J Am Vet Med Assoc 1961 ; 138 : 151 - 152 . Ellis WA , Cassels JÁ , Doyle J. Genital leptospirosis in bull . Vet Rec 1986 ; 118 : 333 . Sambrook J. , Fritsch , EF , Maniatis T. . Molecular cloning: a laboratory manual . Cold Spring Harbor Press , New York , 1989 : 957 . Schönberg A. . Studies on the effect of antibiotic substances on leptospires and their cultivation from material with a high bacterial count . Zent Bakt Parasit Infekt Hyg 1981 ; 249 : 400 - 406 . Kiktenko VS , Balashov NG , Rodina VN. Leptospirosis infection though insemination of animals . J Hyg Epidemiol Microbiol Immunol 1976 ; 20 : 207 - 213 . Ellis WA , O'Brien JJ , Cassells JA . Role of cattle in the maintenance of Leptospira interrogans serotype hardjo infection in Northern Ireland . Vet Rec 1981 ; 108 : 555 - 557 . 1986; 118 1980; 28 1976; 20 1985; 39 1989; 50 1986; 119 1987; 31 1988; 25 1982; 20 1981; 249 1980; 93 1982; 1/4 1982 1961; 138 1981; 108 1989; 27 1992; 62 1983; 44 1989 1992; 30 e_1_2_2_13_2 e_1_2_2_12_2 e_1_2_2_5_2 e_1_2_2_22_2 e_1_2_2_6_2 Sambrook J. (e_1_2_2_14_2) 1989 e_1_2_2_21_2 e_1_2_2_20_2 Mérien F. (e_1_2_2_11_2) 1992; 30 Eaglesome MD (e_1_2_2_2_2) 1992; 62 Schönberg A. (e_1_2_2_16_2) 1981; 249 Schönberg A. (e_1_2_2_17_2) 1980; 93 Thiermann AB (e_1_2_2_9_2) 1983; 44 e_1_2_2_19_2 Ellis WA (e_1_2_2_4_2) 1985; 39 Sleight SD (e_1_2_2_7_2) 1961; 138 (e_1_2_2_15_2) 1987; 31 (e_1_2_2_3_2) 1982; 1 Eys GJJM (e_1_2_2_18_2) 1989; 27 Kiktenko VS (e_1_2_2_8_2) 1976; 20 Bolin CA (e_1_2_2_10_2) 1989; 50 |
| References_xml | – start-page: 957 year: 1989 – volume: 118 start-page: 11 year: 1986 end-page: 13 article-title: Prevalence of serovar in the genital tracts of non‐pregnant cattle publication-title: Vet Rec – volume: 25 start-page: 221 year: 1988 end-page: 235 article-title: Isolamento de leptospiras a partir do tecido renal de hamsters experimental‐mente infectados com serotipo . Emprego das técnicas da pipeta Pasteur e das diluições seriadas em meios de cultura de Fletcher tratado com 5‐fluor‐uracil ou o sulfato de neomicina publication-title: Rev Fac Med Vet Zootec USP – volume: 20 start-page: 207 year: 1976 end-page: 213 article-title: Leptospirosis infection though insemination of animals publication-title: J Hyg Epidemiol Microbiol Immunol – volume: 44 start-page: 2244 year: 1983 end-page: 2245 article-title: Bovine leptospirosis: bacteriologic versus serologic diagnosis of cows at slaughter publication-title: Am J Vet Res – volume: 31 start-page: 136 year: 1987 end-page: 140 article-title: Guimarães MC, Santa Rosa CA, Vasconcellos SA, Oliveira JEAS, Cõrtes JA. Diagnóstico da leptospirose em bovinos doadores de sêmen. Pesquisa de aglutininas séricas e tentativa de isolamento do agente a partir de amostras de sêmen publication-title: Rev Bras Reprod Anim – volume: 119 start-page: 84 year: 1986 end-page: 86 article-title: Problems associated with the serological diagnosis of serovar infection in bovine populations publication-title: Vet Rec – volume: 93 start-page: 166 year: 1980 end-page: 171 article-title: Methods for the detection of leptospires in sperm samples of boar publication-title: Berl Munch Tierarztl Wochenschr – volume: 20 start-page: 178 year: 1982 end-page: 180 article-title: Bovine leptospirosis: serological findings in aborting cows publication-title: Vet Rec – volume: 108 start-page: 555 year: 1981 end-page: 557 article-title: Role of cattle in the maintenance of serotype infection in Northern Ireland publication-title: Vet Rec – volume: 39 start-page: 296 year: 1985 end-page: 98 article-title: Excretion of serovar following calving or abortion publication-title: Res Vet Sci – volume: 118 start-page: 333 year: 1986 article-title: Genital leptospirosis in bull publication-title: Vet Rec – volume: 138 start-page: 151 year: 1961 end-page: 152 article-title: Transmission of bovine leptospirosis by coition and artificial insemination ‐ A preliminary report publication-title: J Am Vet Med Assoc – volume: 62 start-page: 743 year: 1992 end-page: 75 article-title: Microbial agents associated with bovine genital tract infections and semen. Part I. and publication-title: Vet Bul – volume: 27 start-page: 2258 year: 1989 end-page: 2262 article-title: Detection of leptospires in urine by polymerase chain reaction publication-title: J Clin Microbiol – volume: 50 start-page: 1001 year: 1989 end-page: 1003 article-title: Comparison of three techniques to detect serovar type ‐bovis in bovine urine publication-title: Am J Vet Res – start-page: 171 year: 1982 – volume: 28 start-page: 174 year: 1980 end-page: 177 article-title: The use of a vaccine to prevent leptospiruria in cattle exposed to natural challenge with serovar publication-title: N Z Vet J – volume: 1/4 start-page: 21 year: 1982 end-page: 25 article-title: Guimarães MC, Côrtes JA, Vasconcellos SA, Ito FH. Epidemiologia e controle da leptospirose em bovinos. Papel do portador e seu controle terapêutico publication-title: Comun Cient Fac Med Vet Zootec USP – volume: 30 start-page: 2219 year: 1992 end-page: 2224 article-title: Polymerase chain reaction for detection of spp. in clinical samples publication-title: J Clin Microbiol – volume: 249 start-page: 400 year: 1981 end-page: 406 article-title: Studies on the effect of antibiotic substances on leptospires and their cultivation from material with a high bacterial count publication-title: Zent Bakt Parasit Infekt Hyg – volume: 1 start-page: 21 year: 1982 ident: e_1_2_2_3_2 article-title: Guimarães MC, Côrtes JA, Vasconcellos SA, Ito FH. Epidemiologia e controle da leptospirose em bovinos. Papel do portador e seu controle terapêutico publication-title: Comun Cient Fac Med Vet Zootec USP – volume: 93 start-page: 166 year: 1980 ident: e_1_2_2_17_2 article-title: Methods for the detection of leptospires in sperm samples of boar publication-title: Berl Munch Tierarztl Wochenschr contributor: fullname: Schönberg A. – volume: 27 start-page: 2258 year: 1989 ident: e_1_2_2_18_2 article-title: Detection of leptospires in urine by polymerase chain reaction publication-title: J Clin Microbiol doi: 10.1128/jcm.27.10.2258-2262.1989 contributor: fullname: Eys GJJM – volume: 44 start-page: 2244 year: 1983 ident: e_1_2_2_9_2 article-title: Bovine leptospirosis: bacteriologic versus serologic diagnosis of cows at slaughter publication-title: Am J Vet Res contributor: fullname: Thiermann AB – ident: e_1_2_2_12_2 – volume: 31 start-page: 136 year: 1987 ident: e_1_2_2_15_2 article-title: Guimarães MC, Santa Rosa CA, Vasconcellos SA, Oliveira JEAS, Cõrtes JA. Diagnóstico da leptospirose em bovinos doadores de sêmen. Pesquisa de aglutininas séricas e tentativa de isolamento do agente a partir de amostras de sêmen publication-title: Rev Bras Reprod Anim – volume: 62 start-page: 743 year: 1992 ident: e_1_2_2_2_2 article-title: Microbial agents associated with bovine genital tract infections and semen. Part I. Brucella abortus, Leptospira, Campylobacter fetus and Trichomonas foetus publication-title: Vet Bul contributor: fullname: Eaglesome MD – start-page: 957 volume-title: Molecular cloning: a laboratory manual year: 1989 ident: e_1_2_2_14_2 contributor: fullname: Sambrook J. – ident: e_1_2_2_5_2 doi: 10.1136/vr.118.12.333 – ident: e_1_2_2_22_2 doi: 10.1136/vr.108.26.555 – volume: 249 start-page: 400 year: 1981 ident: e_1_2_2_16_2 article-title: Studies on the effect of antibiotic substances on leptospires and their cultivation from material with a high bacterial count publication-title: Zent Bakt Parasit Infekt Hyg contributor: fullname: Schönberg A. – ident: e_1_2_2_19_2 doi: 10.1080/00480169.1980.34741 – volume: 39 start-page: 296 year: 1985 ident: e_1_2_2_4_2 article-title: Excretion of Leptospira interrogans serovar hardjo following calving or abortion publication-title: Res Vet Sci doi: 10.1016/S0034-5288(18)31717-X contributor: fullname: Ellis WA – ident: e_1_2_2_13_2 doi: 10.11606/issn.2318-3659.v25i2p221-235 – ident: e_1_2_2_21_2 doi: 10.1136/vr.119.4.84 – volume: 20 start-page: 207 year: 1976 ident: e_1_2_2_8_2 article-title: Leptospirosis infection though insemination of animals publication-title: J Hyg Epidemiol Microbiol Immunol contributor: fullname: Kiktenko VS – volume: 30 start-page: 2219 year: 1992 ident: e_1_2_2_11_2 article-title: Polymerase chain reaction for detection of Leptospira spp. in clinical samples publication-title: J Clin Microbiol doi: 10.1128/jcm.30.9.2219-2224.1992 contributor: fullname: Mérien F. – ident: e_1_2_2_20_2 doi: 10.1136/vr.110.8.178 – volume: 138 start-page: 151 year: 1961 ident: e_1_2_2_7_2 article-title: Transmission of bovine leptospirosis by coition and artificial insemination ‐ A preliminary report publication-title: J Am Vet Med Assoc contributor: fullname: Sleight SD – ident: e_1_2_2_6_2 doi: 10.1136/vr.118.1.11 – volume: 50 start-page: 1001 year: 1989 ident: e_1_2_2_10_2 article-title: Comparison of three techniques to detect Leptospira interrogans serovar hardjo type hardjo‐bovis in bovine urine publication-title: Am J Vet Res contributor: fullname: Bolin CA |
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| Snippet | Objective In view of the considerable importance of vene real transmission of bovine leptospirosis, the objective of the present study was to compare the... In view of the considerable importance of venereal transmission of bovine leptospirosis, the objective of the present study was to compare the polymerase chain... Objective In view of the considerable importance of vene real transmission of bovine leptospirosis, the objective of the present study was to compare the... OBJECTIVEIn view of the considerable importance of venereal transmission of bovine leptospirosis, the objective of the present study was to compare the... |
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| SubjectTerms | agglutination tests ai bulls Animals Antibodies, Bacterial - blood Brazil Cattle Cattle Diseases - prevention & control Cattle Diseases - transmission cell culture detection Female Insemination, Artificial - veterinary Leptospira Leptospira - isolation & purification leptospirosis Leptospirosis - prevention & control Leptospirosis - transmission Leptospirosis - veterinary PCR polymerase chain reaction Polymerase Chain Reaction - methods Polymerase Chain Reaction - veterinary semen Semen - microbiology Species Specificity |
| Title | Detection of leptospires in bovine semen by polymerase chain reaction |
| URI | https://api.istex.fr/ark:/67375/WNG-P2X2V77K-N/fulltext.pdf https://onlinelibrary.wiley.com/doi/abs/10.1111%2Fj.1751-0813.1999.tb12422.x https://www.ncbi.nlm.nih.gov/pubmed/10028391 https://search.proquest.com/docview/1534812819 https://search.proquest.com/docview/69593516 |
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