High levels of genetic diversity in populations of Iris aphylla L. (Iridaceae), an endangered species in Poland

• Published in: Botanical journal of the Linnean Society Vol. 142; no. 1; pp. 65 - 72
• Main Authors: WRÓBLEWSKA, ADA, BRZOSKO, EMILIA, CZARNECKA, BOŻENNA, NOWOSIELSKI, JAROSŁAW
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01-05-2003
• Subjects: gene flow; isolation; marginal population; RAPDs; steppe plant
• ISSN: 0024-4074; 1095-8339
• DOI: 10.1046/j.1095-8339.2003.00162.x

We used RAPDs (Random Amplified Polymorphic DNAs) to investigate genetic diversity and its partition within and between three populations of Iris aphylla in Poland. Analysis of Molecular Variance (AMOVA) of 84 distinct RAPD multiband genotypes revealed higher variation within populations (77.2%) than genetic differentiation between them (22.8%, P < 0.002). Values of genetic diversity indices (H) were similar in all three sites (0.21–0.24). The differentiation of the populations corresponded to low average gene flow (Nm = 0.81). Our results indicated that genetic diversity was independent of population size. We concluded that although sexual reproduction and gene flow between populations of I. aphylla were very limited, they preserved high levels of genetic diversity. Relatively large number of seeds, which migrated in the past to populations, as well as patterns of reproduction and life history of I. aphylla may explain this situation. © 2003 The Linnean Society of London, Botanical Journal of the Linnean Society, 2003, 142, 65–72.