Increased 8-OHdG levels in the urine, serum, and substantia nigra of hemiparkinsonian rats

• Published in: Brain research Vol. 1133; no. 1; pp. 49 - 52
• Main Authors: Yasuhara, Takao, Hara, Koichi, Sethi, Kapil D, Morgan, John C, Borlongan, Cesario V
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 16-02-2007; Amsterdam Elsevier; New York, NY
• Subjects: 8-OHdG; Adult and adolescent clinical studies; Animals; Biological and medical sciences; Biomarker; Biomarkers - blood; Biomarkers - metabolism; Biomarkers - urine; Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases; Deoxyguanosine - analogs & derivatives; Deoxyguanosine - blood; Deoxyguanosine - metabolism; Deoxyguanosine - urine; Disease Progression; Medical sciences; Neurodegeneration; Neurology; Neurotoxins; Organic mental disorders. Neuropsychology; Oxidative Stress; Oxidopamine; Parkinson's disease; Parkinsonian Disorders - blood; Parkinsonian Disorders - physiopathology; Parkinsonian Disorders - urine; Psychology. Psychoanalysis. Psychiatry; Psychopathology. Psychiatry; Rats; Rats, Sprague-Dawley; Substantia Nigra - metabolism; Substantia Nigra - physiopathology; Time Factors; Up-Regulation; Oxidative stress; Biomarker; Parkinson's disease; AD; 8-hydroxy-2′-deoxyguanosine; 6-OHDA; 6-hydroxydopamine; PD; multiple system atrophy patients; Neurodegeneration; CSF; cerebrospinal fluid; MSA; Alzheimer's disease; 8-OHdG; Urine; Biological fluid; Nervous system diseases; Rat; Rodentia; Central nervous system; Biological marker; Parkinson disease; Cerebral disorder; Vertebrata; Mammalia; Locus niger; Animal; Central nervous system disease; Serum; Degenerative disease; Degeneration; Extrapyramidal syndrome
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/j.brainres.2006.11.072

Abstract 8-Hydroxy-2′-deoxyguanosine (8-OHdG), the predominant marker of oxidative DNA damage, may be a good biomarker for monitoring the progression of Parkinson's disease (PD). Unfortunately, there are no basic laboratory data examining 8-OHdG levels in animal models of PD. In this study, we demonstrate that rats lesioned with 6-hydroxydopamine (6-OHDA) in the medial forebrain bundle display significantly elevated 8-OHdG levels in urine, serum, and substantia nigra, but not cerebrospinal fluid and striatum, compared to sham controls. These increments in 8-OHdG levels were detected at 2 days, but not at 7 days after the lesion suggesting that oxidative stress is restricted to the acute phase of 6-OHDA neurotoxicity. The present results support 8-OHdG as a biomarker that may aid both in the diagnosis and in the documentation of progression in PD.