Chromosome aberrations detected by FISH and correlation with cell survival after irradiation at various dose-rates and after bromodeoxyuridine radiosensitization

• Published in: International journal of radiation biology Vol. 78; no. 3; pp. 203 - 210
• Main Authors: Kreder, N. Castro, Bree, C. Van, Franken, N. A. P., Haveman, J.
• Format: Journal Article
• Language: English
• Published: London Informa UK Ltd 01-03-2002; Taylor & Francis
• Subjects: Biological and medical sciences; Bromodeoxyuridine - pharmacology; Cell Survival - drug effects; Cell Survival - genetics; Cell Survival - radiation effects; Chromosome Aberrations - drug effects; Chromosome Aberrations - radiation effects; Dose-Response Relationship, Radiation; Humans; In Situ Hybridization, Fluorescence; Medical sciences; Radiation therapy and radiosensitizing agent; Radiation Tolerance - drug effects; Treatment with physical agents; Treatment. General aspects; Tumor Cells, Cultured; Tumor Stem Cell Assay; Tumors; Chromosomal aberration; Human; Lung disease; Cell culture; Squamous cell carcinoma; Respiratory disease; Malignant tumor; Radiotherapy; In vitro; Survival; Bronchopulmonary; Radiosensitizing agent; Broxuridine; Fluorescence in situ hybridization; Established cell line; Cytogenetics; Bronchus disease; Molecular biology; Dose
• ISSN: 0955-3002; 1362-3095
• DOI: 10.1080/09553000110104074

Purpose : To determine whether measurement of chromosome aberrations by fluorescence in situ hybridization (FISH) predicts cell survival after irradiation at different dose-rates and after radiosensitization by bromodeoxyurdine (BrdU) in a lung carcinoma cell line. Materials and methods : The human lung carcinoma cell line SW1573 was irradiated at high dose-rate (HDR: 0.8 Gy min -1) or at pulsed low dose-rate (p-LDR: average dose-rate 1 Gy h -1) with or without radiosensitization by bromodeoxyuridine (BrdU). Cell survival was determined by clonogenic assay. Chromosome aberrations (colour junctions) were measured by whole-chromosome FISH of chromosome 2 and 18 and were scored according to the PAINT method. Results : Clear radiosensitization by BrdU was observed both after HDR and p-LDR irradiation. Chromosome 18 was more radiosensitive than chromosome 2. There was a good correlation between induction of colour junctions and cell survival both after HDR and p-LDR irradiation and after radiosensitization by BrdU. Conclusions : Determination of chromosome aberrations by FISH can predict cell survival after different dose-rates and after radiosensitization by BrdU