Increasing specimen coverage using digital whole-mount breast pathology: Implementation, clinical feasibility and application in research

• Published in: Computerized medical imaging and graphics Vol. 35; no. 7; pp. 531 - 541
• Main Authors: Clarke, Gina M, Peressotti, Chris, Constantinou, Paul, Hosseinzadeh, Danoush, Martel, Anne, Yaffe, Martin J
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 01-10-2011
• Subjects: Biomarker distribution; Breast cancer; Breast Neoplasms - pathology; Computers; Diagnostic Imaging - methods; Feasibility Studies; Female; Histological sampling; Humans; Image Interpretation, Computer-Assisted - instrumentation; Image Interpretation, Computer-Assisted - methods; Imaging, Three-Dimensional - instrumentation; Internal Medicine; Microscopy; Other; Radiologic-pathologic correlation; Serial sections; Software; Specimen Handling - methods; User-Computer Interface; Virtual microscopy; Whole-mount sections; Whole-mount sections; Serial sections; Virtual microscopy; Radiologic-pathologic correlation; Histological sampling; Breast cancer; Biomarker distribution
• ISSN: 0895-6111; 1879-0771
• DOI: 10.1016/j.compmedimag.2011.05.002

Abstract Conventional histopathological evaluation is performed on breast specimens using a highly limited sampling of tissues visualized in a two-dimensional (2D) manner although important tumor measurements are three-dimensional. Here we describe a ‘3D’ technique for whole-mount, whole-specimen processing which reduces conformational change and dramatically increases specimen coverage, based on digitizing whole-specimen, whole-mount (up to 12.7 cm × 17.8 cm) serial sections. We describe hardware and software tools for acquiring, viewing and processing the large image datasets (up to 400 GB), validation studies investigating the clinical significance of the additional information gleaned from the 3D approach, and application to radiologic-pathologic correlation and biomarker visualization.