Systemic Identification and Functional Characterization of Common in Fungal Extracellular Membrane Proteins in Lasiodiplodia theobromae

• Published in: Frontiers in plant science Vol. 12; p. 804696
• Main Authors: Peng, Junbo, Wu, Linna, Zhang, Wei, Zhang, Qi, Xing, Qikai, Wang, Xuncheng, Li, Xinghong, Yan, Jiye
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 20-12-2021
• Subjects: cell death; CFEM domain; immune response; Lasiodiplodia theobromae; Plant Science; temperature; temperature; cell death; immune response; CFEM domain; Lasiodiplodia theobromae
• ISSN: 1664-462X; 1664-462X
• DOI: 10.3389/fpls.2021.804696

Plant pathogenic fungi deploy secreted proteins into apoplastic space or intracellular lumen to promote successful infections during plant-pathogen interactions. In the present study, fourteen CFEM domain-containing proteins were systemically identified in
and eight of them were functionally characterized. All eight proteins were confirmed to be secreted into extracellular space by a yeast signal peptide trapping system. The transcriptional levels of most CFEM genes, except for
and
, were significantly elevated during infection. In addition, almost all
genes, apart from
,
, and
, were transcriptionally up-regulated at 35°C in contrast to that at 25°C and 30°C. As two elicitors, LtCFEM1 induced local yellowish phenotype and LtCFEM4 triggered cell death in
leaves. Furthermore, these proteins displayed distinct subcellular localizations when expressed transiently in
. Moreover, two genes,
and
, were found to be spliced alternatively by RT-PCR and sequencing. Therefore, our data suggest that LtCFEM proteins play important roles in multiple aspects, including pathogenicity and plant immune response, which will enhance our understanding of the sophisticated pathogenic mechanisms of plant opportunistic pathogen
.