The MAP Kinase CfPMK1 Is a Key Regulator of Pathogenesis, Development, and Stress Tolerance of Colletotrichum fructicola

The MAP Kinase CfPMK1 Is a Key Regulator of Pathogenesis, Development, and Stress Tolerance of Colletotrichum fructicola

The Ascomycetes fungus causes severe diseases on a wide range of crops, fruits, and vegetables. Its pathogenic mechanisms, however, remain poorly understood. Mitogen-activated protein kinases (MAPKs) are conserved regulators of fungal development and pathogenesis. In this study, a Fus3/Kss1-related...

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Published in:Frontiers in microbiology Vol. 10; p. 1070
Main Authors: Liang, Xiaofei, Wei, Tingyu, Cao, Mengyu, Zhang, Xin, Liu, Wenkui, Kong, Yuanyuan, Zhang, Rong, Sun, Guangyu
Format: Journal Article
Language:English
Published: Switzerland Frontiers Media S.A 21-05-2019
Subjects:
Colletotrichum
Glomerella
MAPK
Microbiology
perithecium
PMK1
sexual reproduction
sexual reproduction
Glomerella
Colletotrichum
MAPK
PMK1
perithecium
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Summary:The Ascomycetes fungus causes severe diseases on a wide range of crops, fruits, and vegetables. Its pathogenic mechanisms, however, remain poorly understood. Mitogen-activated protein kinases (MAPKs) are conserved regulators of fungal development and pathogenesis. In this study, a Fus3/Kss1-related MAPK from was functionally characterized via gene deletion. On potato dextrose agar (PDA) and oatmeal agar media, the gene deletion mutants (Δ ) were slightly reduced in radial growth rate, severely limited in aerial hyphal differentiation and hyphal melanization, and formed deformed perithecia that were smaller in size and more compactly organized relative to wild type. When artificially inoculated on plants, conidia of these mutants failed to differentiate appressoria or penetrate cuticle, and their pathogenicity defect could not be rescued by wounding plant tissue prior to inoculation. On PDA, Δ mutants were hypersensitive to osmotic stresses, but were more tolerant to membrane and cell wall stresses. Genetic complementation rescued all phenotypic changes associated with gene deletion. Based on GFP fusion expression, CfPMK1 protein accumulation was detected at all life stages, and the accumulation level was higher in nascent appressoria relative to conidia. Overall, this study identified CfPMK1 as a key regulator of appressorium and sexual development, pathogenesis, and stress tolerance in .
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This article was submitted to Fungi and Their Interactions, a section of the journal Frontiers in Microbiology
Reviewed by: Zheng Wang, Yale University, United States; Changbin Chen, Institut Pasteur of Shanghai (CAS), China
Edited by: Siu Mui Tsai, University of São Paulo, Brazil
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2019.01070