Decreased CD8 + Lymphocytic Infiltration in Multifocal and Multicentric Glioblastomas

• Published in: Frontiers in oncology Vol. 11; p. 748277
• Main Authors: Wang, Run, Song, Yifu, Hu, Tianhao, Wang, Xiaoliang, Jiang, Yang, Zhang, Di, Yu, Juanhan, Han, Sheng, Kan, Liang
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 27-09-2021
• Subjects: CD8; chemotherapy; Multifocal glioma; Oncology; prognosis; tumor-infiltrating lymphocytes (TILs); Multifocal glioma; tumor-infiltrating lymphocytes (TILs); chemotherapy; prognosis; CD8
• ISSN: 2234-943X; 2234-943X
• DOI: 10.3389/fonc.2021.748277

Multifocal and multicentric glioblastomas (mGBMs) are associated with a poorer prognosis compared to unifocal glioblastoma (uGBM). The presence of CD8
tumor-infiltrating lymphocytes (TILs) is predictive of clinical outcomes in human malignancies. Here, we examined the CD8
lymphocytic infiltration in mGBMs.
The clinical data of 57 consecutive IDH wildtype primary mGBM patients with histopathological diagnoses were retrospectively reviewed. CD8
TILs were quantitatively evaluated by immunohistochemical staining. The survival function of CD8
TILs was assessed by Kaplan-Meier analysis and Cox proportional hazard models.
No significant difference in the concentration of CD8
TILs was observed among foci from the same patient (P>0.150). The presence of CD8
TILs was similar between multifocal and multicentric GBMs (P=0.885). The concentration of CD8
TILs was significantly lower in mGBMs than in uGBMs (P=0.002). In mGBM patients, the CD8
TIL level was associated with preoperative KPS (P=0.018). The median overall survival (OS) of the 57 mGBMs was 9 months. A low CD8
TIL level (multivariate HR 4.404, 95% CI 1.954-9.926, P=0.0004) was an independent predictor of poor OS, while postoperative temozolomide chemotherapy (multivariate HR 6.076, 95% CI 2.330-15.842, P=0.0002) was independently associated with prolonged OS in mGBMs.
Decreased CD8
TIL levels potentially correlate with unfavorable clinical outcome in mGBMs, suggesting an influence of the local immuno-microenvironment on the progression of mGBMs.