Pathological remodelling of colonic wall following dopaminergic nigrostriatal neurodegeneration

• Published in: Neurobiology of disease Vol. 139; p. 104821
• Main Authors: Pellegrini, Carolina, Ippolito, Chiara, Segnani, Cristina, Dolfi, Amelio, Errede, Mariella, Virgintino, Daniela, Fornai, Matteo, Antonioli, Luca, Garelli, Francesca, Nericcio, Anna, Colucci, Rocchina, Cerri, Silvia, Blandini, Fabio, Blandizzi, Corrado, Bernardini, Nunzia
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-06-2020; Elsevier
• Subjects: 6-hydroxydopamine; Animals; Anoctamin-1; Collagen deposition; Colon; Colon - metabolism; Colon - pathology; Dopamine - metabolism; Dopaminergic Neurons - pathology; Fibrosis; Gastrointestinal Diseases - metabolism; Gastrointestinal Motility; Histochemistry; Immunofluorescence; Immunohistochemistry; Intestinal dysfunctions; Male; Morphological remodelling; Oxidopamine; Parkinson Disease - metabolism; Parkinson Disease - pathology; Parkinson's disease; Rat model; Rats; Rats, Sprague-Dawley; Substantia Nigra; Immunohistochemistry; GI; Parkinson's disease; Morphological remodelling; PCNA; Collagen deposition; Intestinal dysfunctions; SR/FG; ICCs; SMC; 6-OHDA; Rat model; PPP; α-SMA; 6-hydroxydopamine; PD; S100; Colon; Immunofluorescence; TMEM16A/ANO1; Histochemistry
• ISSN: 0969-9961; 1095-953X
• DOI: 10.1016/j.nbd.2020.104821

Patients with Parkinson's disease (PD) are often characterized by functional gastrointestinal disorders. Such disturbances can occur at all stages of PD and precede the typical motor symptoms of the disease by many years. However, the morphological alterations associated with intestinal disturbances in PD are undetermined. This study examined the remodelling of colonic wall in 6-hydroxydopamine (6-OHDA)-induced PD rats.
8 weeks after 6-OHDA injection animals were sacrificed. Inflammatory infiltrates, collagen deposition and remodelling of intestinal epithelial barrier and tunica muscularis in the colonic wall were assessed by histochemistry, immunohistochemistry, immunofluorescence and western blot analysis.
6-OHDA rats displayed significant alterations of colonic tissues as compared with controls. Signs of mild inflammation (eosinophil infiltration) and a transmural deposition of collagen fibres were observed. Superficial colonic layers were characterized by severe morphological alterations. In particular, lining epithelial cells displayed a reduced claudin-1 and transmembrane 16A/Anoctamin 1 (TMEM16A/ANO1) expression; goblet cells increased their mucin expression; colonic crypts were characterized by an increase in proliferating epithelial cells; the density of S100-positive glial cells and vimentin-positive fibroblast-like cells was increased as well. Several changes were found in the tunica muscularis: downregulation of α-smooth muscle actin/desmin expression and increased proliferation of smooth muscle cells; increased vimentin expression and proliferative phenotype in myenteric ganglia; reduction of interstitial cells of Cajal (ICCs) density.
A pathological remodelling occurs in the colon of 6-OHDA rats. The main changes include: enhanced fibrotic deposition; alterations of the epithelial barrier; activation of mucosal defense; reduction of ICCs. These results indicate that central nigrostriatal denervation is associated with histological changes in the large bowel at mucosal, submucosal and muscular level. These alterations might represent morphological correlates of digestive symptoms in PD.
•6-OHDA-induced central dopaminergic neurodegeneration elicits bowel alterations.•Such changes include colonic inflammation and gut mucosal barrier impairment.•In addition, colonic wall fibrotic remodelling occur in 6-OHDA-rats.•6-OHDA also elicited a decrease in interstitial cells of Cajal density.•Such alterations could account for digestive symptoms observed in PD patients.