Veratridine increases the survival of retinal ganglion cells in vitro

• Published in: Brazilian journal of medical and biological research Vol. 30; no. 12; pp. 1467 - 1470
• Main Authors: Pereira, S P, Araujo, E G
• Format: Journal Article
• Language: English
• Published: Brazil Associação Brasileira de Divulgação Científica 01-12-1997
• Subjects: Animals; Animals, Newborn; BIOLOGY; Cell Survival - drug effects; development; In Vitro Techniques; MEDICINE, RESEARCH & EXPERIMENTAL; natural cell death; Rats; Rats, Inbred Strains; retina; Retina - drug effects; retinal ganglion cells; Retinal Ganglion Cells - drug effects; veratridine; Veratridine - pharmacology; veratridine; natural cell death; development; retinal ganglion cells; retina
• ISSN: 0100-879X; 1414-431X; 0100-879X; 1414-431X
• DOI: 10.1590/S0100-879X1997001200014

Neuronal cell death is an important phenomenon involving many biochemical pathways. This degenerative event has been studied to understand how the cells activate the mechanisms that lead to self-destruction. Target cells and afferent cells play a relevant role in the regulation of natural cell death. We studied the effect of veratridine (1.5, 3.0, 4.5 and 6.0 microM) on the survival of neonatal rat retinal ganglion cells in vitro. Veratridine (3.0 microM), a well-known depolarizing agent that opens the Na+ channel, promoted a two-fold increase in the survival of retinal ganglion cells kept in culture for 48 h. This effect was dose-dependent and was blocked by 1.0 microM tetrodotoxin (a classical voltage-dependent Na+ channel blocker) and 30.0 microM flunarizine (a Na+ and Ca2+ channel blocker). These results indicate that electrical activity is also important for the maintenance of retinal ganglion cell survival in vitro.