Amelioration of Alcohol Induced Gastric Ulcers Through the Administration of Lactobacillus plantarum APSulloc 331261 Isolated From Green Tea

• Published in: Frontiers in microbiology Vol. 11; p. 420
• Main Authors: Park, Haryung, Cho, Donghyun, Huang, Eunchong, Seo, Ju Yeon, Kim, Wan Gi, Todorov, Svetoslav Dimitrov, Ji, Yosep, Holzapfel, Wilhelm Heinrich
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 17-03-2020
• Subjects: Akkermansia; alcohol induced gastric ulcers; gut microbiome; Lactobacillus plantarum; Microbiology; short chain fatty acids; Akkermansia; alcohol induced gastric ulcers; Lactobacillus plantarum; gut microbiome; short chain fatty acids
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2020.00420

Gastric inflammation is an indication of gastric ulcers and possible other underlying gastric malignancies. Epidemiological studies have revealed that several Asian countries, including South Korea, suffer from a high incidence of gastric diseases derived from high levels of stress, alcoholic consumption, pyloric infection and usage of non-steroidal anti-inflammatory drugs (NSAIDs). Clinical treatments of gastric ulcers are generally limited to proton pump inhibitors that neutralize the stomach acid, and the application of antibiotics for
eradication, both of which are known to have a negative effect on the gut microbiota. The potential of probiotics for alleviating gastrointestinal diseases such as intestinal bowel syndrome and intestinal bowel disease receives increasing scientific interest. Probiotics may support the amelioration of disease-related symptoms through modulation of the gut microbiota without causing dysbiosis. In this study the potential of
APSulloc 331261 (GTB1
), isolated from green tea, was investigated for alleviating gastric inflammation in an alcohol induced gastric ulcer murine model (positive control). Treatment with the test strain significantly influenced the expression of pro-inflammatory and anti-inflammatory biomarkers, interleukin 6 (IL6) and interleukin 10 (IL10), of which the former was down- and the latter up-regulated when the alcohol induced mice were treated with the test strain. This positive effect was also indicated by less severe gastric morphological changes and the histological score of the gastric tissues. A significant increase in the abundance of
within the GTB1
treated group compared to the positive control group also correlated with a decrease in the ratio of acetate over propionate. The increased levels of propionate in the GTB1
group appear to result from the impact of the test strain on the microbial population and the resulting metabolic activities. Moreover, there was a significant increase in beta-diversity in the group that received GTB1
over that of the alcohol induced control group.