Isolation of Candida dubliniensis from denture wearers

• Published in: Journal of medical microbiology Vol. 58; no. 7; pp. 959 - 962
• Main Authors: Gasparoto, Thais Helena, Dionisio, Thiago Jose, de Oliveira, Carine Ervolino, Porto, Vinicius Carvalho, Gelani, Valeria, Santos, Carlos Ferreira, Campanelli, Ana Paula, Lara, Vanessa Soares
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01-07-2009; Society for General Microbiology
• Subjects: Adult; Aged; Aged, 80 and over; Biological and medical sciences; Candida - classification; Candida - genetics; Candida - isolation & purification; Candidiasis, Oral - microbiology; Dentures - adverse effects; DNA, Fungal - chemistry; DNA, Fungal - isolation & purification; Female; Fundamental and applied biological sciences. Psychology; Humans; Infectious diseases; Male; Medical sciences; Microbiology; Middle Aged; Miscellaneous; Mycology; Stomatitis, Denture - microbiology; Ascomycota; Fungi; Isolation; Microbiology; Candida dubliniensis
• ISSN: 0022-2615; 1473-5644
• DOI: 10.1099/jmm.0.008391-0

1 Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil
2 Department of Stomatology (Pathology), Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil
3 Department of Prosthodontics, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil
Correspondence Vanessa Soares Lara vanessa{at}fob.usp.br
Received November 30, 2008
Accepted March 4, 2009
Candida albicans is considered the most important Candida species able to cause oral infections in denture wearers. In recent years, Candida dubliniensis has emerged as a pathogenic yeast in humans. The close phenotypic similarities of C. albicans and C. dubliniensis have led to the misidentification of these species. In this work, our aim was to verify through PCR the presence of C. dubliniensis in palate and maxillary denture samples from 112 denture wearers presenting with or without denture-related stomatitis (DRS). C. dubliniensis was isolated at low rates from both palate (5.3 % and 10.7 %) and maxillary denture (5.3 % and 8.9 %) samples from wearers regardless of the presence of the disease. However, when C. dubliniensis was detected in individuals with DRS, it was always associated with C. albicans . In addition, our results showed that C. albicans was the most commonly identified candidal species in maxillary denture and hard palate samples from DRS patients (78.5 % and 89.2 %, respectively) as well as from controls (31.2 % and 28.5 %, respectively). In conclusion, C. dubliniensis was detected in the oral environment of denture wearers. The association of C. dubliniensis with C. albicans occurred in approximately 10 % of the DRS cases.
These authors contributed equally to this paper.