IL-4 induces adherence of human eosinophils and basophils but not neutrophils to endothelium. Association with expression of VCAM-1

• Published in: The Journal of immunology (1950) Vol. 148; no. 4; pp. 1086 - 1092
• Main Authors: Schleimer, RP, Sterbinsky, SA, Kaiser, J, Bickel, CA, Klunk, DA, Tomioka, K, Newman, W, Luscinskas, FW, Gimbrone, MA, Jr, McIntyre, BW
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Assoc Immnol 15-02-1992; American Association of Immunologists
• Subjects: Analysis of the immune response. Humoral and cellular immunity; Antibodies, Monoclonal - immunology; Basophils - drug effects; Basophils - physiology; Biological and medical sciences; Cell Adhesion; Cell Adhesion Molecules - analysis; Cell Adhesion Molecules - physiology; E-Selectin; Endothelium, Vascular - physiology; Eosinophils - drug effects; Eosinophils - physiology; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Humans; Immunobiology; Intercellular Adhesion Molecule-1; Interleukin-1 - physiology; Interleukin-4 - pharmacology; Lymphokines, interleukins ( function, expression); Neutrophils - drug effects; Neutrophils - physiology; Receptors, Very Late Antigen - analysis; Regulatory factors and their cellular receptors; Tumor Necrosis Factor-alpha - physiology; Vascular Cell Adhesion Molecule-1; Human; Interleukin 4; Cytokine; Cell adhesion molecule; Neutrophil; Mechanism of action; Adhesion; Biological activity; Basophil; Eosinophil; Comparative study; Endothelium
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.148.4.1086

The present studies were performed to explore potentially selective mechanisms of leukocyte adhesion in an attempt to understand how preferential recruitment of eosinophils and basophils might occur during allergic and other inflammatory reactions. Stimulation of human vascular endothelial cells for 24 h with IL-4 (30 to 1,000 U/ml) induced adhesion for eosinophils (up to approximately four-fold of control) and basophils (up to approximately twofold of control) but not neutrophils (less than 125% of control). Analysis of endothelial expression of adhesion molecules by flow cytometry revealed that IL-4 treatment induced vascular cell adhesion molecule-1 (VCAM-1) expression without significantly affecting the expression of other adhesion molecules, namely endothelial-leukocyte adhesion molecule-1 (ELAM-1) or intercellular adhesion molecule-1 (ICAM-1). The concentration-response curve for IL-4-induced VCAM-1 expression paralleled that for adhesion. Endothelial cells stimulated with IL-4 expressed adhesive properties for eosinophils by 3 h; the response increased steadily during a 24-h time course study. Eosinophils and basophils adhered to plates coated with a recombinant form of VCAM-1. This adhesion was blocked with antibodies to VCAM-1 but not ELAM-1. mAb directed against either VCAM-1 or VLA-4 inhibited (by approximately 75%) the binding of eosinophils and basophils to IL-4-stimulated endothelial cells. Because VLA-4 and VCAM-1 have been demonstrated to bind to each other in other adhesion systems, these results suggest that IL-4 stimulates eosinophil and basophil adhesion by inducing endothelial cell expression of VCAM-1 which binds to eosinophil and basophil VLA-4. The lack of expression of VLA-4 on neutrophils and the failure of IL-4 to stimulate neutrophil adherence support this conclusion. It is proposed that local release of IL-4 in vivo in allergic diseases or after experimental allergen challenge may partly explain the enrichment of eosinophils and basophils (vs neutrophils) observed in these situations.