Agro-industrial oily wastes as substrates for PHA production by the new strain Pseudomonas aeruginosa NCIB 40045: Effect of culture conditions

• Published in: Biochemical engineering journal Vol. 26; no. 2; pp. 159 - 167
• Main Authors: Fernández, D., Rodríguez, E., Bassas, M., Viñas, M., Solanas, A.M., Llorens, J., Marqués, A.M., Manresa, A.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Lausanne Elsevier B.V 01-11-2005; Amsterdam Elsevier Science; New York, NY
• Subjects: Biological and medical sciences; Biotechnology; Fundamental and applied biological sciences. Psychology; Low aeration rates; PHA; Pseudomonas aeruginosa; Waste-free fatty acids; PHA; Low aeration rates; Waste-free fatty acids; Pseudomonadales; Free fatty acid; Aeration; Substrate; Industrial waste; Production; Bacteria; Pseudomonadaceae; Pseudomonas aeruginosa; Culture
• ISSN: 1369-703X; 1873-295X
• DOI: 10.1016/j.bej.2005.04.022

Production of poly(3-hydroxyalkaonates) (PHA) by
Pseudomonas aeruginosa 42A2 from agro-industrial oily wastes was studied. PHA accumulation, throughout the cell cycle, was observed as intracellular accumulation associated to polyphosphate granules. A 54.6% PHA accumulation was obtained when technical oleic acid (TOA) was used as carbon source. Molecular weight of the polymer was 54.7 Da. The polymer was amorphous, with glass transition at −47.5
°C and thermal degradation at 293
°C. PHA production and monomer composition were affected by
K
La and temperature. The most relevant characteristic of the polymer produced at low aeration rates (
K
La, 0.06
s
−1) were the unusual C
14:2 (13%) and the increase of C
12:1 (42.2%). The highest amount of unsaturated monomers was found in the polymer produced at 18
°C (64.4%).
PHA accumulation ranged between 66.1% when waste-free fatty acids from soybean oil (WFFA) were used as carbon substrate, 29.4% when waste frying oil (WFO) was used and 16.8% when glucose was used. Depending on the substrate supplied a wide range of components was observed. Major saturated or unsaturated components of the polymer found were C
10:0, C
12:0 and C
8:0 or C
12:1 and C
14:1, respectively. When glucose was used as carbon substrate C
9:0, C
11:0 and C
16:0 were found.