In vitro effects of Cassava ( Manihot esculenta) leaf extracts on four development stages of Haemonchus contortus

• Published in: Veterinary parasitology Vol. 173; no. 1; pp. 85 - 92
• Main Authors: Marie-Magdeleine, C., Udino, L., Philibert, L., Bocage, B., Archimede, H.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 11-10-2010; Elsevier
• Subjects: Adult worm motility; Animals; Anthelmintics - chemistry; Anthelmintics - pharmacology; Chemical and Process Engineering; Condensed tannins; Egg hatch assay; Engineering Sciences; Food engineering; Gastrointestinal nematodes; Haemonchus - drug effects; Haemonchus - growth & development; Larval development; Larval migration; Life Sciences; Manihot - chemistry; Ovum - drug effects; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Leaves - chemistry; Egg hatch assay; Adult worm motility; Gastrointestinal nematodes; Larval migration; Larval development; Condensed tannins; larval development; egg hatch assay; condensed tannin; adult worm motility; gastrointestinal nematode; larval migration
• ISSN: 0304-4017; 1873-2550
• DOI: 10.1016/j.vetpar.2010.06.017

Three extracts (aqueous, methanolic and dichloromethane) of
Manihot esculenta (Cassava) leaf were tested
in vitro on four development stages of
Haemonchus contortus using egg hatch assay (EHA), larval development assay (LDA), L3 migration inhibition assay (LMI) and adult worm motility assay (AWM). Compared to the negative control, significant effects (
P
<
0.0001) were observed for the methanolic extract of leaf against larval development (57.6% ±7.6), with a dose dependent effect. These results suggest that Cassava possess anthelmintic activity against
H. contortus. The active principles responsible for the activity could be the terpenoids and condensed tannin compounds present in the leaf.