Evaluation of the interaction between sodium hypochlorite and several formulations containing chlorhexidine and its effect on the radicular dentin-SEM and push-out bond strength analysis

• Published in: Microscopy research and technique Vol. 77; no. 1; pp. 17 - 22
• Main Authors: Graziele Magro, Miriam, Carlos Kuga, Milton, Regina Victorino, Keli, Antonio Vázquez-Garcia, Fernando, Javier Aranda-Garcia, Arturo, Batista Faria-Junior, Norberto, Faria, Gisele, Luis Shinohara, André
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01-01-2014; Wiley Subscription Services, Inc
• Subjects: Aged; Anti-Bacterial Agents - chemistry; Bonding strength; Chlorhexidine - analogs & derivatives; Chlorhexidine - chemistry; Cuspid - chemistry; Cuspid - ultrastructure; Debris; Dental Stress Analysis; Dentin - chemistry; Dentin - ultrastructure; endodontics; Female; Formulations; Humans; Irrigation; Male; Microscopy, Electron, Scanning; Middle Aged; Peritoneal Lavage - instrumentation; root canal; Root Canal Irrigants - chemistry; Root Canal Preparation - instrumentation; Scanning electron microscopy; Sealers; Smear; smear layer; Sodium Hypochlorite - chemistry; Teeth; endodontics; scanning electron microscopy; debris; smear layer; root canal
• ISSN: 1059-910X; 1097-0029
• DOI: 10.1002/jemt.22307

ABSTRACT
The aim of the current study was to evaluate the presence of debris and smear layer after endodontic irrigation with different formulations of 2% chlorhexidine gluconate (CHX) and its effects on the push‐out bond strength of an epoxy‐based sealer on the radicular dentin. One hundred extracted human canines were prepared to F5 instrument and irrigated with 2.5% sodium hypochlorite and 17% ethylenediaminetetraacetic acid. Fifty teeth were divided into five groups (n = 10), according to the final irrigation protocol with different 2% CHX formulations: G1 (control, no final rinse irrigation), G2 (CHX solution), G3 (CHX gel), G4 (Concepsis), and G5 (CHX Plus). In sequence, the specimens were submitted to scanning electron microscopy (SEM) analysis, in the cervical‐medium and medium‐apical segments, to evaluate the presence of debris and smear layer. The other 50 teeth were treated equally to a SEM study, but with the root canals filled with an epoxy‐based endodontic sealer and submitted to a push‐out bond strength test, in the cervical, middle, and apical thirds. G2, G3, G4, and G5 provided higher precipitation of the debris and smear layer than G1 (P < 0.05), but these groups were similar to each other (P > 0.05), in both segments. The values obtained in the push out test did not differ between groups, independent of the radicular third (P > 0.05). The CHXs formulations caused precipitation of the debris and smear layer on the radicular dentin, but these residues did not interfere in the push‐out bond strength of the epoxy‐based sealer. Microsc. Res. Tech. 77:17–22, 2014. © 2013 Wiley Periodicals, Inc.