Active intranuclear movement of herpesvirus capsids

Although small molecules diffuse rapidly through the interphase nucleus, recent reports indicate that nuclear diffusion is limited for particles that are larger than 100 nm in diameter. Given the apparent size limits to nuclear diffusion, there is some debate as to whether the movement of large part...

Full description

Saved in:

Bibliographic Details
Published in:	Nature cell biology Vol. 7; no. 4; pp. 429 - 431
Main Authors:	Baines, Joel D, Forest, Thomas, Barnard, Sandra
Format:	Journal Article
Language:	English
Published:	England Nature Publishing Group 01-04-2005
Subjects:	Biological transport, Active Capsid - metabolism Capsid - physiology Cell Nucleus - metabolism Cell Nucleus - physiology Cells Diffusion Herpes simplex virus 1 Herpes viruses Herpesvirus Herpesvirus 1, Human - genetics Herpesvirus 1, Human - physiology Herpesviruses Humans Particle Size Physiological aspects Sodium Temperature United States
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Although small molecules diffuse rapidly through the interphase nucleus, recent reports indicate that nuclear diffusion is limited for particles that are larger than 100 nm in diameter. Given the apparent size limits to nuclear diffusion, there is some debate as to whether the movement of large particles should be attributed to diffusion or to active transport. Here, we show that 125 nm-diameter herpes simplex virus 1 (HSV-1) capsids are actively transported within infected nuclei. Movement is directed, temperature- and energy-dependent, sensitive to the putative myosin inhibitor 2,3-butanedione monoxime (BDM) and to actin depolymerization with latrunculin-A, but insensitive to actin depolymerization with cytochalasin-D.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	1465-7392 1476-4679 1476-4679
DOI:	10.1038/ncb1243