Optimizing parameters for correlative immunogold localization by video- enhanced light microscopy, high-voltage transmission electron microscopy, and field emission scanning electron microscopy

Correlative video-enhanced light microscopy, high-voltage transmission electron microscopy, and low-voltage high resolution scanning electron microscopy were used to examine the binding of colloidal gold-labeled fibrinogen to platelet surfaces. Optimal conditions for the detection of large (18 nm) a...

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Bibliographic Details
Published in:	The journal of histochemistry and cytochemistry Vol. 38; no. 12; pp. 1781 - 1785
Main Authors:	Simmons, SR, Pawley, JB, Albrecht, RM
Format:	Journal Article
Language:	English
Published:	Los Angeles, CA Histochemical Soc 01-12-1990 SAGE Publications
Subjects:	Blood Platelets - metabolism Blood Platelets - ultrastructure Fibrinogen - metabolism Gold Image Processing, Computer-Assisted Immunohistochemistry Microscopy, Electron - methods Microscopy, Electron, Scanning
Online Access:	Get full text
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Summary:	Correlative video-enhanced light microscopy, high-voltage transmission electron microscopy, and low-voltage high resolution scanning electron microscopy were used to examine the binding of colloidal gold-labeled fibrinogen to platelet surfaces. Optimal conditions for the detection of large (18 nm) and small (3 nm) gold particles are described.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0022-1554 1551-5044
DOI:	10.1177/38.12.2254644