Adaptation of GAL4 activators for GAL4 enhancer trapping in zebrafish

An enhancer trap‐based GAL4‐UAS system in zebrafish requires strong GAL4 activators with minimal adverse effects. However, the activity of yeast GAL4 is too low in zebrafish, while a fusion protein of the GAL4 DNA‐binding domain and the VP16 activation domain is toxic to embryonic development, even...

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Bibliographic Details
Published in:	Developmental dynamics Vol. 238; no. 3; pp. 641 - 655
Main Authors:	Ogura, Eri, Okuda, Yuichi, Kondoh, Hisato, Kamachi, Yusuke
Format:	Journal Article
Language:	English
Published:	New York Wiley‐Liss, Inc 01-03-2009
Subjects:	Adaptation, Biological - genetics Animals Danio rerio Embryo, Nonmammalian - embryology Embryo, Nonmammalian - metabolism Enhancer trap GAL4 activators GAL4VP16 GAL4‐UAS Gene Expression Regulation, Developmental Genes, Reporter - genetics Genetic Techniques Genetic Vectors - genetics Sleeping Beauty transposon Trans-Activators - genetics Transcription Factors - genetics Transcription Factors - metabolism Transcription, Genetic - genetics Transposases - genetics Transposases - metabolism zebrafish Zebrafish - genetics Zebrafish - metabolism
Online Access:	Get full text
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