Long-lived fluorescence of homopolymeric guanine-cytosine DNA duplexes

Long-lived fluorescence of homopolymeric guanine-cytosine DNA duplexes

The fluorescence spectrum of the homopolymeric double helix poly(dG) x poly(dC) is dominated by emission decaying on the nanosecond time-scale, as previously reported for the alternating homologue poly(dGdC) x poly(dGdC). Thus, energy trapping over long periods of time is a common feature of GC dupl...

Full description

Saved in:
Bibliographic Details
Published in:Photochemical & photobiological sciences Vol. 9; no. 9; p. 1193
Main Authors: Vayá, Ignacio, Changenet-Barret, Pascale, Gustavsson, Thomas, Zikich, Dragoslav, Kotlyar, Alexander B, Markovitsi, Dimitra
Format: Journal Article
Language:English
Published: England 01-01-2010
Subjects:
Base Pairing
DNA - chemistry
Poly C - chemistry
Poly G - chemistry
Spectrometry, Fluorescence
Thermodynamics
Time Factors
Online Access:Get more information
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The fluorescence spectrum of the homopolymeric double helix poly(dG) x poly(dC) is dominated by emission decaying on the nanosecond time-scale, as previously reported for the alternating homologue poly(dGdC) x poly(dGdC). Thus, energy trapping over long periods of time is a common feature of GC duplexes which contrast with AT duplexes. The impact of such behaviour on DNA photodamage needs to be evaluated.
ISSN:1474-9092
DOI:10.1039/c0pp00201a