Flow-Seq Evaluation of Translation Driven by a Set of Natural Escherichia coli 5′-UTR of Variable Length

Flow-seq is a method that combines fluorescently activated cell sorting and next-generation sequencing to deduce a large amount of data about translation efficiency from a single experiment. Here, we constructed a library of fluorescent protein-based reporters preceded by a set of 648 natural 5′-unt...

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Bibliographic Details
Published in:	International journal of molecular sciences Vol. 23; no. 20; p. 12293
Main Authors:	Komarova, Ekaterina S., Slesarchuk, Anna N., Rubtsova, Maria P., Osterman, Ilya A., Tupikin, Alexey E., Pyshnyi, Dmitry V., Dontsova, Olga A., Kabilov, Marsel R., Sergiev, Petr V.
Format:	Journal Article
Language:	English
Published:	Basel MDPI AG 14-10-2022 MDPI
Subjects:	Binding sites Cloning E coli Efficiency Escherichia coli Flow-seq Fluorescence Gene expression Libraries Next-generation sequencing Protein structure Proteins ribosome Secondary structure Translation translation efficiency untranslated region
Online Access:	Get full text
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