Identification and Characterization of Tomato Mutants Affected in the Rx-Mediated Resistance to PVX Isolates

• Published in: Molecular plant-microbe interactions Vol. 25; no. 3; pp. 341 - 354
• Main Authors: STURBOIS, Bénédicte, DUBRANA-OURABAH, Marie-Pierre, GOMBERT, Julie, LASSEUR, Bertrand, MACQUET, Audrey, FAURE, Chantal, BENDAHMANE, Abdelhafid, BAURES, Isabelle, CANDRESSE, Thierry
• Format: Journal Article
• Language: English
• Published: St. Paul, MN APS Press 01-03-2012; American Phytopathological Society; The American Phytopathological Society
• Subjects: Biochemistry, Molecular Biology; Biological and medical sciences; Capsid Proteins - genetics; Capsid Proteins - metabolism; Chromosome Mapping; Disease Resistance - genetics; Disease Resistance - immunology; DNA, Plant - genetics; Fundamental and applied biological sciences. Psychology; Genes, Plant - genetics; Host-Pathogen Interactions; Life Sciences; Lycopersicon esculentum; Mutagenesis; Mutation; Nicotiana - virology; Phenotype; Phytopathology. Animal pests. Plant and forest protection; Plant Diseases - immunology; Plant Diseases - virology; Plant Leaves - virology; Plants, Genetically Modified; Potato virus X; Potexvirus - pathogenicity; Potexvirus - physiology; RNA, Plant - genetics; RNA, Viral - genetics; Solanum lycopersicum - genetics; Solanum lycopersicum - immunology; Solanum lycopersicum - virology; Species Specificity; Transcription, Genetic; Transgenes - genetics; Virulence; Resistance; Vegetable crop; Dicotyledones; Angiospermae; Lycopersicon esculentum; Isolate; Spermatophyta; Identification; Solanaceae; Mutation; MICRO-TOM; RESISTANCE MEDIEE; PVX; GENETIQUE; BIOLOGIE MOLECULAIRE
• ISSN: 0894-0282; 1943-7706
• DOI: 10.1094/MPMI-07-11-0181

Five tomato mutants affected in the Rx-mediated resistance against Potato virus X (PVX) were identified by screening a mutagenized population derived from a transgenic, Rx1-expressing 'Micro-Tom' line. Contrary to their parental line, they failed to develop lethal systemic necrosis upon infection with the virulent PVX-KH2 isolate. Sequence analysis and quantitative reverse-transcription polymerase chain reaction experiments indicated that the mutants are not affected in the Rx1 transgene or in the Hsp90, RanGap1 and RanGap2, Rar1 and Sgt1 genes. Inoculation with the PVX-CP4 avirulent isolate demonstrated that the Rx1 resistance was still effective in the mutants. In contrast, the virulent PVX-KH2 isolate accumulation was readily detectable in all mutants, which could further be separated in two groups depending on their ability to restrict the accumulation of PVX-RR, a mutant affected at two key positions for Rx1 elicitor activity. Finally, transient expression of the viral capsid protein elicitor indicated that the various mutants have retained the ability to mount an Rx1-mediated hypersensitive response. Taken together, the results obtained are consistent with a modification of the specificity or intensity of the Rx1-mediated response. The five Micro-Tom mutants should provide very valuable resources for the identification of novel tomato genes affecting the functioning of the Rx gene.