Characterization of lytic enzyme activities of Lactobacillus gasseri with special reference to autolysis

• Published in: International journal of food microbiology Vol. 96; no. 3; pp. 273 - 279
• Main Authors: Yokoi, Ken-ji, Kawasaki, Ken-Ichi, Taketo, Akira, Kodaira, Ken-Ichi
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15-11-2004; Elsevier
• Subjects: autolysis; Autolytic activity; bacterial proteins; Bacteriolysis - drug effects; Bacteriolysis - physiology; Biological and medical sciences; Culture Media; Dose-Response Relationship, Drug; enzyme activity; enzyme substrates; Food industries; Food microbiology; Fundamental and applied biological sciences. Psychology; Hydrogen-Ion Concentration; lactic acid bacteria; Lactobacillus - drug effects; Lactobacillus - enzymology; Lactobacillus - radiation effects; Lactobacillus gasseri; Lytic enzyme; microbial activity; mitomycin; Molecular Weight; Probiotics; prophage induction; screening; Sodium Chloride - pharmacology; turbidity; ultraviolet radiation; Ultraviolet Rays; Zymogram; Zymogram; Autolytic activity; Lytic enzyme; Lactobacillus gasseri; Autolysis; Characterization; Enzyme; Reference; Bacteria; Lactobacillaceae
• ISSN: 0168-1605; 1879-3460
• DOI: 10.1016/j.ijfoodmicro.2004.03.021

Lactobacillus gasseri JCM 1130 and JCM 1131
T exhibited autolytic activity in agar containing autoclaved cells of each strain as substrate. By zymogram analysis of JCM 1131
T, two lytic bands with apparent molecular masses of 54.5 and 35 kDa, were detected. Similarly, JCM 1130 yielded two lytic bands with apparent molecular masses of 35 and 33.5 kDa. In simple buffers as well, JCM 1131
T suffered a drastic decrease in cell turbidity, but JCM 1130 did not undergo the decrease. The optimal pH for autolysis of JCM 1131
T was in the range of 6.0–7.0, and the lysis was completely inhibited at pH 4–5. The lysis of JCM 1131
T was suppressed by NaCl, in a concentration-dependent way. When subjected to UV irradiation or mitomycin C (MMC) treatment, cultures of both strains elicited conspicuous turbidity decrease after 2–4 h of growth, suggesting the occurrence of prophage induction. The 35-kDa lytic band of JCM 1131
T and the 33.5-kDa protein of JCM 1130 were considerably increased by UV irradiation.