Analysis of cell fusion induced by bovine coronavirus infection

Polykaryon formation in bovine fetal spleen (BFS) cells infected with bovine coronavirus L9 occurred only in media supplemented with trypsin. A single 1 to 2 h trypsin treatment 10 h and later after infection induced formation of polykaryons. Trypsin treatment at pH 7.5 and 8.0 induced polykaryons w...

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Bibliographic Details
Published in:	Archives of virology Vol. 103; no. 1/2; pp. 27 - 33
Main Authors:	Payne, H.R, Storz, J
Format:	Journal Article
Language:	English
Published:	Wien Springer 01-01-1988 New York, NY Springer-Verlag
Subjects:	Animals antibodies Antibodies, Viral Biological and medical sciences Cattle cell fusion Cell Fusion - drug effects Cell Line Coronaviridae Coronaviridae - physiology Fundamental and applied biological sciences. Psychology Humans Hydrogen-Ion Concentration Microbiology Original Papers Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Trypsin - pharmacology Virology Cell culture Bovine coronavirus Bovine Multinucleate cell Cell fusion Host virus relation Virus Vertebrata Trypsin Coronavirus Mammalia PH Artiodactyla Coronaviridae Ungulata
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Summary:	Polykaryon formation in bovine fetal spleen (BFS) cells infected with bovine coronavirus L9 occurred only in media supplemented with trypsin. A single 1 to 2 h trypsin treatment 10 h and later after infection induced formation of polykaryons. Trypsin treatment at pH 7.5 and 8.0 induced polykaryons while treatments at lower or higher pH levels did not. Cell fusion activity was partially suppressed by the presence of antibody.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0304-8608 1432-8798
DOI:	10.1007/BF01319806