Expression of single-chain antibody-barstar fusion in plants

We successfully cloned and expressed a single-chain antibody (425scFv), that is directed to human epidermal growth factor receptor HER1 (EGFR) in transgenic tobacco plants as a fusion with bacterial barstar gene (425scFv-barstar). Plant-produced recombinant 425scFv-barstar was recovered using barsta...

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Bibliographic Details
Published in:	Biochimie Vol. 89; no. 1; pp. 31 - 38
Main Authors:	Semenyuk, Ekaterina G., Stremovskiy, Oleg A., Edelweiss, Evelina F., Shirshikova, Olga V., Balandin, Taras G., Buryanov, Yaroslav I., Deyev, Sergey M.
Format:	Journal Article
Language:	English
Published:	France Elsevier Masson SAS 2007
Subjects:	Bacterial Proteins - genetics Barnase Barstar Blotting, Western Cloning, Molecular Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay ErbB Receptors - immunology Flow Cytometry HER1 (EGFR) Humans Immunoglobulin Fragments - biosynthesis Immunoglobulin Fragments - immunology Microscopy, Fluorescence Nicotiana - genetics Plants, Genetically Modified Protein Engineering - methods Receptor, ErbB-2 Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - immunology Single-chain Fv antibody Transgenic plants TSP Barnase IMAC Single-chain Fv antibody scFv HRP Barstar Transgenic plants HER1 (EGFR) ELISA
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