Soybean [Glycine max] BRU1 encodes a functional xyloglucan endotransglycosylase that is highly expressed in inner epicotyl tissues during brassinosteroid-promoted elongation

• Published in: Plant and cell physiology Vol. 39; no. 1; pp. 124 - 130
• Main Authors: Oh, M.H. (North Carolina State Univ., Raleigh (USA)), Romanow, W.G, Smith, R.C, Zamski, E, Sasse, J, Clouse, S.D
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01-01-1998
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; BRASINOSTEROIDES; Brassinosteroid; BRASSINOSTEROIDE; BRASSINOSTEROIDS; BRUl; CELL DIFFERENTIATION; cell growth; Classical and quantitative genetics. Population genetics. Molecular genetics; developmental stages; DIFERENCIACION CELULAR; DIFFERENCIATION CELLULAIRE; Elongation; enzyme activity; EPICOTILOS; EPICOTYLE; EPICOTYLS; EXPRESION GENICA; EXPRESSION DES GENES; Fundamental and applied biological sciences. Psychology; GENE EXPRESSION; Generalities. Genetics. Plant material; genes; Genes. Genome; genetic code; genetic regulation; Genetics and breeding of economic plants; GLICOSILTRANSFERASAS; GLYCINE MAX; GLYCOSYLTRANSFERASE; GLYCOSYLTRANSFERASES; hexosyltransferases; localization; Molecular and cellular biology; Molecular genetics; parenchyma; phloem; plant anatomy; PLANT TISSUES; Soybean; TEJIDOS VEGETALES; TISSU VEGETAL; transcription (genetics); XET; Xyloglucan endotransglycosylase; xyloglucans; Epicotyl; Enzyme; Transferases; Glycosyltransferases; Tissue specificity; Gene expression; Glycine max; Xyloglucan:xyloglucosyl transferase; Grain legume; Regulation(control); Brassinosteroid; Leguminosae; Gene; Dicotyledones; Phytosterol; Angiospermae; Hexosyltransferases; Spermatophyta; Elongation
• ISSN: 0032-0781; 1471-9053
• DOI: 10.1093/oxfordjournals.pcp.a029283

Brassinosteroids promote soybean epicotyl elongation and regulate expression of BRU1, a gene with homology to xyloglucan endotransglycosylases (XETs). Recombinant BRU1 protein possesses XET activity and in situ hybridization reveals highest BRU1 transcript accumulation in inner epicotyl tissue, particularly the phloem and paratracheary parenchyma cells. These results suggest a role for BRU1 in vascular development in addition to cell elongation.