invasive Mimosa in India does not adopt the symbionts of its native relatives

• Published in: Annals of botany Vol. 112; no. 1; pp. 179 - 196
• Main Authors: Gehlot, Hukam Singh, Tak, Nisha, Kaushik, Muskan, Mitra, Shubhajit, Chen, Wen-Ming, Poweleit, Nicole, Panwar, Dheeren, Poonar, Neetu, Parihar, Rashmita, Tak, Alkesh, Sankhla, Indu Singh, Ojha, Archana, Rao, Satyawada Rama, Simon, Marcelo F, Reis Junior, Fabio Bueno dos, Perigolo, Natalia, Tripathi, Anil K, Sprent, Janet I, Young, J. Peter W, James, Euan K, Gyaneshwar, Prasad
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01-07-2013
• Subjects: Agricultural Inoculants - genetics; Alphaproteobacteria - genetics; Alphaproteobacteria - isolation & purification; Bacteria; Biodiversity; Burkholderia; Burkholderia - genetics; Burkholderia - isolation & purification; Cupriavidus; Cupriavidus - genetics; Cupriavidus - isolation & purification; Desert soils; electron microscopy; Ensifer; genes; Genes, Bacterial; hosts; India; indigenous species; Introduced Species; Legumes; Microbiology; microsymbionts; Mimosa; Mimosa - microbiology; monophyly; nodulation; Nodules; Original; Phylogeny; Plant Roots - genetics; Plant Roots - microbiology; Rhizobium; Rhizobium - physiology; rhizosphere; ribosomal RNA; RNA, Ribosomal, 16S - genetics; soil; Soil fertility; South America; Symbionts; Symbiosis; Brazil; India; South America; Cupriavidus; Betaproteobacteria; Mimosa pudica; arid regions; rhizobia; Mimosa hamata; Mimosa himalayana; Thar Desert; nodulation; Burkholderia; Ensifer; bacterial symbionts; nitrogen fixation
• ISSN: 0305-7364; 1095-8290
• DOI: 10.1093/aob/mct112

Background and AimsThe large monophyletic genus Mimosa comprises approx. 500 species, most of which are native to the New World, with Central Brazil being the main centre of radiation. All Brazilian Mimosa spp. so far examined are nodulated by rhizobia in the betaproteobacterial genus Burkholderia. Approximately 10 Mya, transoceanic dispersal resulted in the Indian subcontinent hosting up to six endemic Mimosa spp. The nodulation ability and rhizobial symbionts of two of these, M. hamata and M. himalayana, both from north-west India, are here examined, and compared with those of M. pudica, an invasive species.MethodsNodules were collected from several locations, and examined by light and electron microscopy. Rhizobia isolated from them were characterized in terms of their abilities to nodulate the three Mimosa hosts. The molecular phylogenetic relationships of the rhizobia were determined by analysis of 16S rRNA, nifH and nodA gene sequences.Key ResultsBoth native Indian Mimosa spp. nodulated effectively in their respective rhizosphere soils. Based on 16S rRNA, nifH and nodA sequences, their symbionts were identified as belonging to the alphaproteobacterial genus Ensifer, and were closest to the ‘Old World’ Ensifer saheli, E. kostiensis and E. arboris. In contrast, the invasive M. pudica was predominantly nodulated by Betaproteobacteria in the genera Cupriavidus and Burkholderia. All rhizobial strains tested effectively nodulated their original hosts, but the symbionts of the native species could not nodulate M. pudica.ConclusionsThe native Mimosa spp. in India are not nodulated by the Burkholderia symbionts of their South American relatives, but by a unique group of alpha-rhizobial microsymbionts that are closely related to the ‘local’ Old World Ensifer symbionts of other mimosoid legumes in north-west India. They appear not to share symbionts with the invasive M. pudica, symbionts of which are mostly beta-rhizobial.