Biological characterisation of Australian isolates of chicken anaemia agent

• Published in: Australian veterinary journal Vol. 68; no. 6; p. 199
• Main Authors: Connor, T J, McNeilly, F, Firth, G A, McNulty, M S
• Format: Journal Article
• Language: English
• Published: England 01-06-1991
• Subjects: Anemia - microbiology; Anemia - veterinary; Animals; Antibodies, Monoclonal - immunology; Antigens, Viral - analysis; Australia; Cell Line; Chickens; Cytopathogenic Effect, Viral; DNA Viruses - immunology; DNA Viruses - pathogenicity; DNA Viruses - physiology; Fluorescent Antibody Technique; Neutralization Tests; Poultry Diseases - microbiology; Specific Pathogen-Free Organisms; Australia
• ISSN: 0005-0423
• DOI: 10.1111/j.1751-0813.1991.tb03192.x

Three Australian isolates of chicken anaemia agent (CAA) resisted treatment at 70 degrees C for 5 min and chloroform treatment. Although minor antigenic differences were detected using monoclonal antibodies to CAA, the Australian isolates were indistinguishable from the reference Cux-1 and Gifu-1 isolates in cross-immunofluorescence and cross-neutralisation tests employing polyclonal chicken antiserums. The Australian viruses were pathogenic for intramuscularly inoculated 1-day-old SPF chicks, but were less pathogenic for 7-day-old chicks. Thus the Australian isolates of CAA did not differ significantly in these properties from previously characterised CAA isolates from other continents.