Evaluation of renal nerve morphological changes and norepinephrine levels following treatment with novel bipolar radiofrequency delivery systems in a porcine model

• Published in: Journal of hypertension Vol. 32; no. 8; pp. 1678 - 1692
• Main Authors: Cohen-Mazor, Meital, Mathur, Prabodh, Stanley, James R.L, Mendelsohn, Farrell O, Lee, Henry, Baird, Rose, Zani, Brett G, Markham, Peter M, Rocha-Singh, Krishna
• Format: Journal Article
• Language: English
• Published: England Wolters Kluwer Health | Lippincott Williams & Wilkins 01-08-2014; Lippincott Williams & Wilkins
• Subjects: Animals; Blood Pressure; Catheter Ablation - methods; Creatinine - blood; Heart Rate; Kidney - chemistry; Kidney - innervation; Models, Animal; Norepinephrine - analysis; ORIGINAL PAPERS: Therapeutic aspects; Renal Artery - innervation; Renal Artery - pathology; Swine; Sympathectomy - methods; Tyrosine 3-Monooxygenase - analysis
• DOI: 10.1097/HJH.0000000000000236
• EISSN: 1473-5598
• ISSN: 0263-6352

OBJECTIVE:To evaluate the safety and effectiveness of different bipolar radiofrequency system algorithms in interrupting the renal sympathetic nerves and reducing renal norepinephrine in a healthy porcine model.
METHODS:A porcine model (N = 46) was used to investigate renal norepinephrine levels and changes to renal artery tissues and nerves following percutaneous renal denervation with radiofrequency bipolar electrodes mounted on a balloon catheter. Parameters of the radiofrequency system (i.e. electrode length and energy delivery algorithm), and the effects of single and longitudinal treatments along the artery were studied with a 7-day model in which swine received unilateral radiofrequency treatments. Additional sets of animals were used to examine norepinephrine and histological changes 28 days following bilateral percutaneous radiofrequency treatment or surgical denervation; untreated swine were used for comparison of renal norepinephrine levels.
RESULTS:Seven days postprocedure, norepinephrine concentrations decreased proportionally to electrode length, with 81, 60 and 38% reductions (vs. contralateral control) using 16, 4 and 2-mm electrodes, respectively. Applying a temperature-control algorithm with the 4-mm electrodes increased efficacy, with a mean 89.5% norepinephrine reduction following a 30-s treatment at 68°C. Applying this treatment along the entire artery length affected more nerves vs. a single treatment, resulting in superior norepinephrine reduction 28 days following bilateral treatment.
CONCLUSION:Percutaneous renal artery application of bipolar radiofrequency energy demonstrated safety and resulted in a significant renal norepinephrine content reduction and renal nerve injury compared with untreated controls in porcine models.