No association of polymorphisms in the glutathione S-transferase genes with pre-eclampsia, eclampsia and HELLP syndrome in a Turkish population

• Published in: The journal of obstetrics and gynaecology research Vol. 31; no. 3; pp. 236 - 241
• Main Authors: Cetin, Meral, Pinarbasi, Ergun, Percin, Ferda E., Akgün, Egemen, Percin, Sibel, Pinarbasi, Hatice, Gurlek, Fatma, Cetin, Ali
• Format: Journal Article
• Language: English
• Published: Melbourne, Australia Blackwell Science Pty 01-06-2005
• Subjects: Adolescent; Adult; Case-Control Studies; DNA Primers; eclampsia; Eclampsia - genetics; European Continental Ancestry Group - genetics; Female; glutathione S-transferase; Glutathione Transferase - genetics; HELLP syndrome; HELLP Syndrome - genetics; Humans; Middle Aged; Polymerase Chain Reaction; polymorphism; Polymorphism, Genetic; pre-eclampsia; Pre-Eclampsia - genetics; Pregnancy; Turkey - epidemiology; Turkey
• ISSN: 1341-8076; 1447-0756
• DOI: 10.1111/j.1447-0756.2005.00281.x

Aim: There is substantial evidence that genetic factors play a role in pre‐eclampsia. The aim of this study was to determine whether genetic variability in the encoding of genes for glutathione S‐transferase M1 (GSTM1) and glutathione S‐transferase T1 (GSTT1) contributes to individual differences in susceptibility to pre‐eclampsia, eclampsia, or hemolysis, elevated liver enzymes, and low platelets (HELLP syndrome).
Methods: A total of 221 women with pre‐eclampsia, eclampsia and HELLP syndrome and 147 healthy female controls were genotyped for GSTM1 and GSTT1 polymorphisms by polymerase chain reaction (PCR). Statistical evaluation of differences in polymorphic rates was carried out using χ2 analysis.
Results: This study included 140 pre‐eclamptic, 33 eclamptic and 48 HELLP syndrome cases and 147 healthy controls. The frequencies for the GSTM1 null genotype were 58%, 45%, and 60% for pre‐eclampsia, eclampsia, and HELLP syndrome, respectively, and in controls it was 55%. The distribution of the GSTT1 null genotype was  22%,  21%,  and  27%  for  pre‐eclampsia,  eclampsia,  and  HELLP  syndrome,  respectively,  and  in  controls  it was 22%. There was no significant association between GSTM1 and GSTT1 polymorphisms and pre‐eclampsia, eclampsia, and HELLP syndrome.
Conclusion: Our data do not support a role for polymorphisms of the GSTM1 and GSTT1 genes in the pathogenesis of pre‐eclampsia, eclampsia and HELLP syndrome.