Mannan-Modified Solid Lipid Nanoparticles for Targeted Gene Delivery to Alveolar Macrophages

• Published in: Pharmaceutical research Vol. 27; no. 8; pp. 1584 - 1596
• Main Authors: Yu, Wangyang, Liu, Chunxi, Liu, Yu, Zhang, Na, Xu, Wenfang
• Format: Journal Article
• Language: English
• Published: Boston Boston : Springer US 01-08-2010; Springer US; Springer; Springer Nature B.V
• Subjects: active targeting; Animals; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; Biomedical Engineering and Bioengineering; Biomedicine; cationic solid lipid nanoparticles; Cell Line; Cell Line, Tumor; Cell Survival; Concanavalin A - administration & dosage; Drug Delivery Systems; gene delivery; Gene therapy; General pharmacology; Genetic Therapy - methods; Ligands; Lipids; Lipids - administration & dosage; Lung cancer; Macrophages, Alveolar - metabolism; Male; mannan-based PE-grafted ligand; Mannans - administration & dosage; Mannans - chemistry; Medical Law; Medical sciences; Mice; Microscopy, Electron, Transmission; Molecular Structure; Nanoparticles; Nanoparticles - administration & dosage; Nanoparticles - chemistry; Pharmaceutical sciences; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Pharmacology/Toxicology; Pharmacy; Phosphatidylethanolamines - administration & dosage; Rats; Rats, Wistar; Research Paper; surface modification; gene delivery; cationic solid lipid nanoparticles; active targeting; surface modification; mannan-based PE-grafted ligand; Pharmaceutical technology; Targeting; Ligand; Targeted therapy; Surface treatment; Genetic transfer; Target; Cations; Gene therapy; Macrophage
• ISSN: 0724-8741; 1573-904X
• DOI: 10.1007/s11095-010-0149-z

Purpose Cationic solid lipid nanoparticles (SLN) have established themselves during the past decades. They can efficiently bind DNA directly via ionic interaction and mediate gene transfection. One major problem with SLN is the lack of cell-targeting ability. In the present study, a mannan-based PE-grafted ligand was synthesized and used for the surface modification of DNA-loaded cationic SLN to prepare Man-SLN-DNA. Methods For in vitro test, the cytotoxicity and transfection investigation was carried out on murine macrophage cell line RAW 264.7. For in vivo evaluation, Man-SLN-DNA was delivered into the lung of the rats, and the alveolar macrophages (AM) were isolated for the fluorescence determination of transfection efficiency. Results When compared with non-modified SLN-DNA and Lipofectamine 2000-DNA, Man-SLN-DNA produced the highest gene expressions, especially in vivo. Conclusion These results demonstrated the active targeting ability of this kind of mannan-modified DNA-loaded vehicles, which may have great potential for targeted gene delivery.