Similarity of copper and technetium binding sites in human IgG

Kits for direct labeling of IgG with 99mTc were used without modification for the preparation of [67Cu]IgG. The IgG was pre-treated to generate thiolate groups which would bind 67Cu. The direct labeling of reduced IgG with 67Cu was highly efficient, resulting in approx. 95% 67Cu binding. Non-reduced...

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Bibliographic Details
Published in:	International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology Vol. 19; no. 7; p. 797
Main Authors:	Zamora, P O, Mercer-Smith, J A, Marek, M J, Schulte, L D, Rhodes, B A
Format:	Journal Article
Language:	English
Published:	England 01-10-1992
Subjects:	Binding Sites Binding, Competitive Chromatography, High Pressure Liquid Copper - metabolism gamma-Globulins - metabolism Humans Immunoglobulin G - metabolism Technetium - metabolism
Online Access:	Get more information
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Summary:	Kits for direct labeling of IgG with 99mTc were used without modification for the preparation of [67Cu]IgG. The IgG was pre-treated to generate thiolate groups which would bind 67Cu. The direct labeling of reduced IgG with 67Cu was highly efficient, resulting in approx. 95% 67Cu binding. Non-reduced IgG (negative control) had labeling efficiencies of less than 10%. IgG pre-exposed to Cu(II) had reduced amounts of 99mTc bound to it. The results demonstrate a direct relationship between copper- and 99mTc-binding sites in IgG.
ISSN:	0883-2897
DOI:	10.1016/0883-2897(92)90142-L