The modulatory effects of ellagic acid and vitamin E succinate on TCDD-induced oxidative stress in different brain regions of rats after subchronic exposure

• Published in: Journal of biochemical and molecular toxicology Vol. 18; no. 4; pp. 196 - 203
• Main Authors: Hassoun, Ezdihar A., Vodhanel, Jennifer, Abushaban, Ahmed
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 2004
• Subjects: Animals; Antioxidants - pharmacology; Body Weight - drug effects; Brain; Brain - drug effects; Brain - metabolism; Cerebral Cortex; DNA Damage; DNA Single Strand Breaks; Drug Administration Schedule; Ellagic Acid; Ellagic Acid - pharmacology; Female; Hippocampus; Lipid Peroxidation; Oxidative Stress; Oxidative Stress - drug effects; Polychlorinated Dibenzodioxins - antagonists & inhibitors; Polychlorinated Dibenzodioxins - toxicity; Rats; Rats, Inbred Strains; Rats, Sprague-Dawley; Subchronic Toxicity; Superoxide Anion; Superoxides - metabolism; TCDD; Tocopherols; Vitamin E - analogs & derivatives; Vitamin E - pharmacology; Vitamin E Succinate
• ISSN: 1095-6670; 1099-0461
• DOI: 10.1002/jbt.20030

The effects of ellagic acid (EA) and vitamin E succinate (VES) on 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin (TCDD)‐induced oxidative stress in different brain regions of rats have been studied after subchronic exposure to the compounds. TCDD was administered to groups of rats at a dose of 46 ng/kg/day for 90 days. EA and VES were administered to groups of rats, either separately or simultaneously with TCDD, every other day for 90 days. At the end of the treatment period, animals were sacrificed and brains were dissected to cerebral cortex (Cc), hippocampus (H), cerebellum (C), and brain stem (Bs), and were assayed for production of superoxide anion (SA), lipid peroxidation (LP), and DNA single‐strand breaks (SSBs). While TCDD administration to rats resulted in significant production of SA, LP, and DNA SSBs in Cc and H, simultaneous administration of VES or EA with the xenobiotics resulted in significant protection against those effects. The results also indicate that VES provided a better protyection against TCDD‐induced effects in brains when compared with EA. © 2003 Wiley Periodicals, Inc. J Biochem Mol Toxicol 18:196–203, 2003; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.20030