Detection of Rare Mutations by Routine Analysis of KRAS, NRAS, and BRAF Oncogenes
Molecular genetic analysis of KRAS , NRAS , and BRAF genes was carried out in order to develop an optimal algorithm for detection of minor mutations. We analyzed 35 melanoma and 33 colorectal cancer specimens. Frequent G12D/V/A/C/S mutations were detected in KRAS . The most frequent BRAF mutation in...
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| Published in: | Bulletin of experimental biology and medicine Vol. 162; no. 3; pp. 375 - 378 |
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| Main Authors: | , , , , |
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| Language: | English |
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2017
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| Abstract | Molecular genetic analysis of
KRAS
,
NRAS
, and
BRAF
genes was carried out in order to develop an optimal algorithm for detection of minor mutations. We analyzed 35 melanoma and 33 colorectal cancer specimens. Frequent G12D/V/A/C/S mutations were detected in
KRAS
. The most frequent
BRAF
mutation in melanoma was V600E, the percentage of rare mutations is significant for DNA diagnosis (24%). Identification of rare
BRAF
mutations 1790C→G (L597R), 1798_1799delinsAA (V600K), 1798_1799delinsAG (V600R), and 1799_1800delinsAA (V600E) and
NRAS
mutation 38G→T (G13V) was possible only by Sanger sequencing. The combination of real-time PCR and sequencing can improve analysis sensitivity and ensure concordance of the tested loci with the international recommendations. |
|---|---|
| AbstractList | Molecular genetic analysis of KRAS, NRAS, and BRAF genes was carried out in order to develop an optimal algorithm for detection of minor mutations. We analyzed 35 melanoma and 33 colorectal cancer specimens. Frequent G12D/V/A/C/S mutations were detected in KRAS. The most frequent BRAF mutation in melanoma was V600E, the percentage of rare mutations is significant for DNA diagnosis (24%). Identification of rare BRAF mutations 1790C→G (L597R), 1798_1799delinsAA (V600K), 1798_1799delinsAG (V600R), and 1799_1800delinsAA (V600E) and NRAS mutation 38G→T (G13V) was possible only by Sanger sequencing. The combination of real-time PCR and sequencing can improve analysis sensitivity and ensure concordance of the tested loci with the international recommendations. Molecular genetic analysis of KRAS, NRAS, and BRAF genes was carried out in order to develop an optimal algorithm for detection of minor mutations. We analyzed 35 melanoma and 33 colorectal cancer specimens. Frequent G12D/V/A/C/S mutations were detected in KRAS. The most frequent BRAF mutation in melanoma was V600E, the percentage of rare mutations is significant for DNA diagnosis (24%). Identification of rare BRAF mutations 1790C [right arrow] G (L597R), 1798_1799delinsAA (V600K), 1798_1799delinsAG (V600R), and 1799_1800delinsAA (V600E) and NRAS mutation 38G [right arrow] T (G13V) was possible only by Sanger sequencing. The combination of real-time PCR and sequencing can improve analysis sensitivity and ensure concordance of the tested loci with the international recommendations. Key Words: oncogene; somatic mutation; sequencing Molecular genetic analysis of KRAS, NRAS, and BRAF genes was carried out in order to develop an optimal algorithm for detection of minor mutations. We analyzed 35 melanoma and 33 colorectal cancer specimens. Frequent G12D/V/A/C/S mutations were detected in KRAS. The most frequent BRAF mutation in melanoma was V600E, the percentage of rare mutations is significant for DNA diagnosis (24%). Identification of rare BRAF mutations 1790C arrow right G (L597R), 1798_1799delinsAA (V600K), 1798_1799delinsAG (V600R), and 1799_1800delinsAA (V600E) and NRAS mutation 38G arrow right T (G13V) was possible only by Sanger sequencing. The combination of real-time PCR and sequencing can improve analysis sensitivity and ensure concordance of the tested loci with the international recommendations. Molecular genetic analysis of KRAS, NRAS, and BRAF genes was carried out in order to develop an optimal algorithm for detection of minor mutations. We analyzed 35 melanoma and 33 colorectal cancer specimens. Frequent G12D/V/A/C/S mutations were detected in KRAS. The most frequent BRAF mutation in melanoma was V600E, the percentage of rare mutations is significant for DNA diagnosis (24%). Identification of rare BRAF mutations 1790C[rarr]G (L597R), 1798_1799delinsAA (V600K), 1798_1799delinsAG (V600R), and 1799_1800delinsAA (V600E) and NRAS mutation 38G[rarr]T (G13V) was possible only by Sanger sequencing. The combination of real-time PCR and sequencing can improve analysis sensitivity and ensure concordance of the tested loci with the international recommendations. Molecular genetic analysis of KRAS , NRAS , and BRAF genes was carried out in order to develop an optimal algorithm for detection of minor mutations. We analyzed 35 melanoma and 33 colorectal cancer specimens. Frequent G12D/V/A/C/S mutations were detected in KRAS . The most frequent BRAF mutation in melanoma was V600E, the percentage of rare mutations is significant for DNA diagnosis (24%). Identification of rare BRAF mutations 1790C→G (L597R), 1798_1799delinsAA (V600K), 1798_1799delinsAG (V600R), and 1799_1800delinsAA (V600E) and NRAS mutation 38G→T (G13V) was possible only by Sanger sequencing. The combination of real-time PCR and sequencing can improve analysis sensitivity and ensure concordance of the tested loci with the international recommendations. Molecular genetic analysis of KRAS, NRAS, and BRAF genes was carried out in order to develop an optimal algorithm for detection of minor mutations. We analyzed 35 melanoma and 33 colorectal cancer specimens. Frequent G12D/V/A/C/S mutations were detected in KRAS. The most frequent BRAF mutation in melanoma was V600E, the percentage of rare mutations is significant for DNA diagnosis (24%). Identification of rare BRAF mutations 1790C [right arrow] G (L597R), 1798_1799delinsAA (V600K), 1798_1799delinsAG (V600R), and 1799_1800delinsAA (V600E) and NRAS mutation 38G [right arrow] T (G13V) was possible only by Sanger sequencing. The combination of real-time PCR and sequencing can improve analysis sensitivity and ensure concordance of the tested loci with the international recommendations. |
| Audience | Academic |
| Author | Alekseev, B. Ya Mikhailenko, D. S. Safronova, N. Yu Efremov, G. D. Strelnikov, V. V. |
| Author_xml | – sequence: 1 givenname: D. S. surname: Mikhailenko fullname: Mikhailenko, D. S. email: dimserg@mail.ru organization: N. A. Lopatkin Research Institute of Urology and Intervention Radiology, Affiliated Department of National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Research Center for Medical Genetics – sequence: 2 givenname: G. D. surname: Efremov fullname: Efremov, G. D. organization: N. A. Lopatkin Research Institute of Urology and Intervention Radiology, Affiliated Department of National Medical Research Radiological Center, Ministry of Health of the Russian Federation – sequence: 3 givenname: N. Yu surname: Safronova fullname: Safronova, N. Yu organization: N. A. Lopatkin Research Institute of Urology and Intervention Radiology, Affiliated Department of National Medical Research Radiological Center, Ministry of Health of the Russian Federation – sequence: 4 givenname: V. V. surname: Strelnikov fullname: Strelnikov, V. V. organization: Research Center for Medical Genetics – sequence: 5 givenname: B. Ya surname: Alekseev fullname: Alekseev, B. Ya organization: N. A. Lopatkin Research Institute of Urology and Intervention Radiology, Affiliated Department of National Medical Research Radiological Center, Ministry of Health of the Russian Federation |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/28091917$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1590/S1806-37132015000004531 10.1158/1078-0432.CCR-12-0052 10.1186/s12885-015-1811-y 10.3748/wjg.v20.i29.9732 10.2147/OTT.S39096 10.1371/journal.pone.0107926 10.1200/JCO.2015.63.9674 10.1016/j.jmoldx.2010.10.006 10.18632/oncotarget.5190 10.18632/oncotarget.3804 10.1093/annonc/mdu260 10.12703/P6-108 |
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| References | CR2 De Stefano, Carlomagno (CR3) 2014; 20 CR6 Zheng, Tseng, Chen, Haley, Illei, Gocke, Eshleman, Lin (CR13) 2015; 15 Fariña Sarasqueta, Moerland, de Bruyne, de Graaf, Vrancken, van Lijnschoten, van den Brule (CR4) 2011; 13 Lopes, Vattimo (CR5) 2015; 41 CR12 CR11 Menzies, Haydu, Visintin, Carlino, Howle, Thompson, Kefford, Scolyer, Long (CR7) 2012; 18 Allegra, Rumble, Hamilton, Mangu, Roach, Hantel, Schilsky (CR1) 2016; 34 Spagnolo, Ghiorzo, Orgiano, Pastorino, Picasso, Tornari, Ottaviano, Queirolo (CR10) 2015; 8 Shan, Li, Ma, Zhang (CR9) 2014; 9 Muñoz-Couselo, Garcia, Perez-Garcia, Cebrián, Castán (CR8) 2015; 3 E Muñoz-Couselo (3619_CR8) 2015; 3 GL Lopes (3619_CR5) 2015; 41 3619_CR6 L Shan (3619_CR9) 2014; 9 A Fariña Sarasqueta (3619_CR4) 2011; 13 3619_CR11 3619_CR12 3619_CR2 A Stefano De (3619_CR3) 2014; 20 CJ Allegra (3619_CR1) 2016; 34 F Spagnolo (3619_CR10) 2015; 8 AM Menzies (3619_CR7) 2012; 18 G Zheng (3619_CR13) 2015; 15 |
| References_xml | – volume: 41 start-page: 365 issue: 4 year: 2015 end-page: 375 ident: CR5 article-title: de Castro Junior Gd. Identifying activating mutations in the EGFR gene: prognostic and therapeutic implications in non-small cell lung cancer publication-title: J. Bras. Pneumol doi: 10.1590/S1806-37132015000004531 contributor: fullname: Vattimo – volume: 18 start-page: 3242 issue: 12 year: 2012 end-page: 3249 ident: CR7 article-title: Distinguishing clinicopathologic features of patients with V600E and V600K BRAF-mutant metastatic melanoma publication-title: Clin. Cancer Res. doi: 10.1158/1078-0432.CCR-12-0052 contributor: fullname: Long – volume: 15 start-page: 779 year: 2015 ident: CR13 article-title: Clinical detection and categorization of uncommon and concomitant mutations involving BRAF publication-title: BMC Cancer. doi: 10.1186/s12885-015-1811-y contributor: fullname: Lin – volume: 20 start-page: 9732 issue: 29 year: 2014 end-page: 9743 ident: CR3 article-title: Beyond KRAS: predictive factors of the efficacy of anti-EGFR monoclonal antibodies in the treatment of metastatic colorectal cancer publication-title: World J. Gastroenterol. doi: 10.3748/wjg.v20.i29.9732 contributor: fullname: Carlomagno – ident: CR2 – volume: 8 start-page: 157 year: 2015 end-page: 168 ident: CR10 article-title: BRAF-mutant melanoma: treatment approaches, resistance mechanisms, and diagnostic strategies publication-title: Onco Targets Ther. doi: 10.2147/OTT.S39096 contributor: fullname: Queirolo – ident: CR12 – ident: CR11 – volume: 3 start-page: 207 issue: 15 year: 2015 ident: CR8 article-title: Recent advances in the treatment of melanoma with BRAF and MEK inhibitors publication-title: Ann. Transl. Med. contributor: fullname: Castán – volume: 9 start-page: e107926 issue: 9 year: 2014 ident: CR9 article-title: PCR-based assays versus direct sequencing for evaluating the effect of KRAS status on anti-EGFR treatment response in colorectal cancer patients: a systematic review and meta-analysis publication-title: PLoS ONE doi: 10.1371/journal.pone.0107926 contributor: fullname: Zhang – ident: CR6 – volume: 34 start-page: 179 issue: 2 year: 2016 end-page: 185 ident: CR1 article-title: Extended RAS gene mutation testing in metastatic colorectal carcinoma to predict response to antiepidermal growth factor receptor monoclonal antibody therapy: American society of clinical oncology provisional clinical opinion update 2015 publication-title: J. Clin. Oncol doi: 10.1200/JCO.2015.63.9674 contributor: fullname: Schilsky – volume: 13 start-page: 199 issue: 2 year: 2011 end-page: 205 ident: CR4 article-title: SNaPshot and StripAssay as valuable alternatives to direct sequencing for KRAS mutation detection in colon cancer routine diagnostics publication-title: J. Mol. Diagn doi: 10.1016/j.jmoldx.2010.10.006 contributor: fullname: van den Brule – volume: 41 start-page: 365 issue: 4 year: 2015 ident: 3619_CR5 publication-title: J. Bras. Pneumol doi: 10.1590/S1806-37132015000004531 contributor: fullname: GL Lopes – volume: 13 start-page: 199 issue: 2 year: 2011 ident: 3619_CR4 publication-title: J. Mol. Diagn doi: 10.1016/j.jmoldx.2010.10.006 contributor: fullname: A Fariña Sarasqueta – ident: 3619_CR6 doi: 10.18632/oncotarget.5190 – volume: 9 start-page: e107926 issue: 9 year: 2014 ident: 3619_CR9 publication-title: PLoS ONE doi: 10.1371/journal.pone.0107926 contributor: fullname: L Shan – volume: 34 start-page: 179 issue: 2 year: 2016 ident: 3619_CR1 publication-title: J. Clin. Oncol doi: 10.1200/JCO.2015.63.9674 contributor: fullname: CJ Allegra – ident: 3619_CR2 doi: 10.18632/oncotarget.3804 – volume: 3 start-page: 207 issue: 15 year: 2015 ident: 3619_CR8 publication-title: Ann. Transl. Med. contributor: fullname: E Muñoz-Couselo – volume: 8 start-page: 157 year: 2015 ident: 3619_CR10 publication-title: Onco Targets Ther. doi: 10.2147/OTT.S39096 contributor: fullname: F Spagnolo – ident: 3619_CR12 doi: 10.1093/annonc/mdu260 – volume: 18 start-page: 3242 issue: 12 year: 2012 ident: 3619_CR7 publication-title: Clin. Cancer Res. doi: 10.1158/1078-0432.CCR-12-0052 contributor: fullname: AM Menzies – volume: 15 start-page: 779 year: 2015 ident: 3619_CR13 publication-title: BMC Cancer. doi: 10.1186/s12885-015-1811-y contributor: fullname: G Zheng – ident: 3619_CR11 doi: 10.12703/P6-108 – volume: 20 start-page: 9732 issue: 29 year: 2014 ident: 3619_CR3 publication-title: World J. Gastroenterol. doi: 10.3748/wjg.v20.i29.9732 contributor: fullname: A Stefano De |
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| Snippet | Molecular genetic analysis of
KRAS
,
NRAS
, and
BRAF
genes was carried out in order to develop an optimal algorithm for detection of minor mutations. We... Molecular genetic analysis of KRAS, NRAS, and BRAF genes was carried out in order to develop an optimal algorithm for detection of minor mutations. We analyzed... |
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| SubjectTerms | Algorithms Base Sequence Biomedical and Life Sciences Biomedicine Cell Biology Colorectal cancer Colorectal carcinoma Colorectal Neoplasms - diagnosis Colorectal Neoplasms - genetics Colorectal Neoplasms - pathology DNA Mutational Analysis DNA sequencing Exons Gene mutation Genes Genetic analysis Genetic aspects GTP Phosphohydrolases - genetics Humans Internal Medicine Introns K-Ras protein Laboratory Medicine Melanoma Melanoma - diagnosis Melanoma - genetics Melanoma - pathology Membrane Proteins - genetics Mutation Neoplasm Proteins - genetics Oncogenes Paraffin Embedding Pathology Polymerase chain reaction Proto-Oncogene Proteins B-raf - genetics Proto-Oncogene Proteins p21(ras) - genetics Real-Time Polymerase Chain Reaction Skin Neoplasms - diagnosis Skin Neoplasms - genetics Skin Neoplasms - pathology Tissue Fixation |
| Title | Detection of Rare Mutations by Routine Analysis of KRAS, NRAS, and BRAF Oncogenes |
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