The Role of ApoE Serum Levels and ApoE Gene Polymorphisms in Patients with Laryngeal Squamous Cell Carcinoma

• Published in: Biomolecules (Basel, Switzerland) Vol. 12; no. 8; p. 1013
• Main Authors: Liutkeviciene, Rasa, Auzelyte, Justina, Liutkevicius, Vykintas, Vilkeviciute, Alvita, Gedvilaite, Greta, Vaiciulis, Paulius, Uloza, Virgilijus
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 22-07-2022; MDPI
• Subjects: Alzheimer's disease; Angiogenesis; ApoE; Apolipoprotein E; Biomarkers; Deoxyribonucleic acid; DNA; Enzyme-linked immunosorbent assay; Gene polymorphism; General anesthesia; Genotyping; Inflammation; Laryngeal cancer; laryngeal squamous cell carcinoma; Lung cancer; Lymph nodes; Metastases; Metastasis; Mortality; Otolaryngology; Patients; Polymerase chain reaction; Proteins; rs7412 and rs429358; Serum levels; Single-nucleotide polymorphism; Squamous cell carcinoma; survival rate; Tumorigenesis; Womens health; United States > US
• ISSN: 2218-273X; 2218-273X
• DOI: 10.3390/biom12081013

Recent studies have revealed that the inflammatory ApoE effect may play a significant role in various cancer development. However, this effect has still not been analyzed in patients with laryngeal squamous cell carcinoma (LSCC). In the present study, we evaluated two single nucleotide polymorphisms (SNPs) of ApoE (rs7412 and rs429358) and determined their associations with LSCC development and the LSCC patients’ five-year survival rate. Additionally, we analyzed serum ApoE levels using an enzyme-linked immunosorbent assay. A total of 602 subjects (291 histologically verified LSCC patients and 311 healthy controls) were involved in this study. The genotyping was carried out using the real-time PCR. We revealed that ApoE ε3/ε3 was associated with a 1.7-fold higher probability of developing LSCC (p = 0.001), with 1.7-fold increased odds of developing LSCC without metastasis to the lymph nodes (p = 0.002) and with a 2.0-fold increased odds of developing well-differentiated LSCC (p = 0.008), as well as 1.6-fold increased odds of developing poorly differentiated LSCC development (p = 0.012). The ApoE ε2/ε4 and ε3/ε4 genotypes were associated with a 2.9-fold and 1.5-fold decrease in the likelihood of developing LSCC (p = 0.042; p = 0.037, respectively). ApoE ε3/ε4 was found associated with a 2.4-fold decreased likelihood of developing well-differentiated LSCC (p = 0.013). Conclusion: ApoE ε2/ε4 and ε3/ε4 were found to play a protective role in LSCC development, while ApoE ε3/ε3 may have a risk position in LSCC development.