Viable Deletion Mutants in the Simian Virus 40 Early Region

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 75; no. 9; pp. 4455 - 4459
• Main Authors: Feunteun, Jean, Kress, Michel, Gardes, Maryvonne, Monier, Roger
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 01-09-1978; National Acad Sciences
• Subjects: 3T3 cells; Antigens, Viral - genetics; Cell growth; Cell Line; Cell lines; Cell Transformation, Viral; DNA; DNA Restriction Enzymes - metabolism; Electrophoresis; Enzymes; Gels; Genes, Viral; Messenger RNA; Molecular Weight; Mutation; Simian virus 40 - genetics; Simian virus 40 - immunology; Viral tumor antigens; Viruses
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.75.9.4455

For the purpose of isolating hr-t-like mutants of simian virus 40, we have constructed variants that have lost the unique site for the restriction enzyme Taq I at 0.565. Five mutants have been isolated and characterized by restriction enzyme analysis. All of them produce a normal size T antigen. Four produce a t antigen reduced in size as well as in amount; the fifth one does not seem to make any t antigen at all. The ability of these mutants to transform mouse cells in vitro, as tested by anchorage dependence, is clearly altered; however, the defect is only partial. In the same test, the mutants can complement a tsA mutant for transformation and therefore define a second complementation group in the simian virus 40 early region.