Expression Analysis of MaTGA8 Transcription Factor in Banana and Its Defence Functional Analysis by Overexpression in Arabidopsis

• Published in: International journal of molecular sciences Vol. 22; no. 17; p. 9344
• Main Authors: Lin, Ping, Dong, Tao, Chen, Wenliang, Zou, Niexia, Chen, Yinglong, Li, Yuqing, Chen, Kelin, Wang, Mingyuan, Liu, Jianfu
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01-09-2021; MDPI
• Subjects: Arabidopsis; Bananas; Binding sites; Biosynthesis; Chlorophyll; Disease resistance; Functional analysis; Fusarium oxysporum; Fusarium oxysporum f. sp. cubense race 4; Gene expression; MaNPR1; MaTGA8 transcription factor; Morphogenesis; Plant diseases; Plant resistance; Polymerase chain reaction; Salicylic acid; Signal transduction; Soil microorganisms; Strong interactions (field theory); Transcription factors
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms22179344

TGA transcription factor is a member of the D subfamily of the basic region-leucine zippers (bZIP) family. It is a type of transcription factor that was first identified in plants and is the main regulator in plant development and physiological processes, including morphogenesis and seed formation in response to abiotic and biotic stress and maintaining plant growth. The present study examined the sequence of the MaTGA8 transcription factor, the sequence of which belonged to subfamily D of the bZIP and had multiple cis-acting elements such as the G-box, TCA-element, TGACG-element, and P-box. Quantitative real time polymerase chain reaction (qRT-PCR) analyses showed that MaTGA8 was significantly down-regulated by the soil-borne fungus Fusarium oxysporum f. sp. cubense race 4 (Foc TR4). Under the induction of salicylic acid (SA), MaTGA8 was down-regulated, while different members of the MaNPR1 family responded significantly differently. Among them, MaNPR11 and MaNPR3 showed an overall upward trend, and the expression level of MaNPR4, MaNPR8, and MaNPR13 was higher than other members. MaTGA8 is a nuclear-localized transcription factor through strong interaction with MaNPR11 or weaker interaction with MaNPR4, and it is implied that the MaPR gene can be activated. In addition, the MaTGA8 transgenic Arabidopsis has obvious disease resistance and higher chlorophyll content than the wild-type Arabidopsis with the infection of Foc TR4. These results indicate that MaTGA8 may enhance the resistance of bananas to Foc TR4 by interacting with MaNPR11 or MaNPR4. This study provides a basis for further research on the application of banana TGA transcription factors in Foc TR4 stress and disease resistance and molecular breeding programs.