Optical biosensing of markers of mucosal inflammation

• Published in: Nanomedicine Vol. 40; p. 102476
• Main Authors: Covarrubias-Zambrano, Obdulia, Motamedi, Massoud, Ameredes, Bill T., Tian, Bing, Calhoun, William J., Zhao, Yingxin, Brasier, Allan R., Kalubowilage, Madumali, Malalasekera, Aruni P., Yapa, Asanka S., Wang, Hongwang, Culbertson, Christopher T., Troyer, Deryl L., Bossmann, Stefan H.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-02-2022
• Subjects: Animals; Asthma; Biomarkers; Breath Tests; Inflammation - diagnosis; Iron/iron oxide core/shell nanoparticle; Lung Diseases; Lung inflammation; Mice; Nanodiagnostics; Nanomedicine; Optical biosensor; CCL20; ADAM 33; EBC; IS; Nanodiagnostics; BAL; IL-6; Iron/iron oxide core/shell nanoparticle; HEPES; NE; Lung inflammation; FRET; MMP-8; Nanomedicine; Optical biosensor; COPD
• ISSN: 1549-9634; 1549-9642
• DOI: 10.1016/j.nano.2021.102476

We report the design and adaptation of iron/iron oxide nanoparticle-based optical nanobiosensors for enzymes or cytokine/chemokines that are established biomarkers of lung diseases. These biomarkers comprise ADAM33, granzyme B, MMP-8, neutrophil elastase, arginase, chemokine (C-C motif) ligand 20 and interleukin-6. The synthesis of nanobiosensors for these seven biomarkers, their calibration with commercially available enzymes and cytokines/chemokines, as well as their validation using bronchoalveolar lavage (BAL) obtained from a mouse model of TLR3-mediated inflammation are discussed here. Exhaled Breath Condensate (EBC) is a minimally invasive approach for sampling airway fluid in the diagnosis and management of various lung diseases in humans (e.g., asthma, COPD and viral infections). We report the proof-of-concept of using human EBC in conjunction with nanobiosensors for diagnosis/monitoring airway inflammation. These findings suggest that, with nanosensor technology, human EBC can be utilized as a liquid biopsy to monitor inflammation/remodeling in lung disease.
Optical nanobiosensors (ONBS) are capable of quantitatively analyzing the activities of signature proteases for lung diseases and arginase, as well as the concentrations of selected cytokines/chemokines in serum, bronchoalveolar lavage (BAL) and exhaled breath condensate (EBC). [Display omitted]