Direct electrochemistry of cytochrome bo3 oxidase at a series of gold nanoparticles-modified electrodes

• Published in: Electrochemistry communications Vol. 26; pp. 105 - 108
• Main Authors: Melin, Frederic, Meyer, Thomas, Lankiang, Styven, Choi, Sylvia K., Gennis, Robert B., Blanck, Christian, Schmutz, Marc, Hellwig, Petra
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 01-01-2013; Amsterdam Elsevier; New York, NY
• Subjects: Biological and medical sciences; Direct electron transfer; Electrocatalysis; Electrochemistry; Fundamental and applied biological sciences. Psychology; Gold nanoparticles; Membrane protein; Molecular biophysics; Physical chemistry in biology; Electrocatalysis; Direct electron transfer; Membrane protein; Gold nanoparticles; Gold; Oxygen; Ubiquinol oxidase; Enzyme; Nanoparticle; Bioelectrochemistry; Escherichia coli; Polycrystal; Electron transfer; Transition metal; Surface structure; Electrodes; Transmission electron microscopy; Morphology; Bacteria; Oxidoreductases; Immobilized enzyme; Enterobacteriaceae; Modified material
• ISSN: 1388-2481; 1873-1902
• DOI: 10.1016/j.elecom.2012.10.024

New membrane-protein based electrodes were prepared incorporating cytochrome bo3 from Escherichiacoli and gold nanoparticles. Direct electron transfer between the electrode and the immobilized enzymes was achieved, resulting in an electrocatalytic activity in presence of O2. The size of the gold nanoparticles was shown to be important and smaller particles were shown to reduce the overpotential of the process.
New membrane-protein based electrodes were prepared incorporating cytochrome bo3 from E. coli and gold nanoparticles. Direct electron transfer between the electrode and the immobilized enzymes was achieved, resulting in an electrocatalytic activity in presence of O2. The size of the gold nanoparticles was shown to be important and smaller particles were shown to reduce the overpotential of the process. [Display omitted]
► Direct electron transfer on membrane protein ► Catalytic current of cytochrome oxidase ► Influence of nanoparticle size