Tc-99m-HMPAO uptake by bronchoalveolar cells

• Published in: Annals of nuclear medicine Vol. 17; no. 2; pp. 107 - 113
• Main Authors: Durak, Hatice, Kilinç, Oğuz, Ertay, Türkan, Uçan, Eyüp Sabri, Kargi, Aydanur, Kaya, Gamze Capa, Sis, Banu
• Format: Journal Article
• Language: English
• Published: Japan Springer Nature B.V 01-04-2003
• Subjects: Administration, Inhalation; Aerosols; Alveoli; Bronchi - diagnostic imaging; Bronchi - metabolism; Bronchoalveolar Lavage Fluid - chemistry; Bronchoalveolar Lavage Fluid - cytology; Bronchus; Cigarettes; Correlation; Female; Glutathione; Humans; Inhalation; Injection; Injections, Intravenous; Intravenous administration; Lavage; Leukocytes (neutrophilic); Lung; Lungs; Macrophages; Macrophages, Alveolar - diagnostic imaging; Macrophages, Alveolar - metabolism; Male; Middle Aged; Mucus; Nuclear medicine; Pulmonary Alveoli - diagnostic imaging; Pulmonary Alveoli - metabolism; Radionuclide Imaging; Radiopharmaceuticals - administration & dosage; Radiopharmaceuticals - pharmacokinetics; Respiration; Smoking; Smoking - metabolism; Statistics as Topic; Technetium Tc 99m Exametazime - administration & dosage; Technetium Tc 99m Exametazime - pharmacokinetics; Toxicity
• ISSN: 0914-7187; 1864-6433
• DOI: 10.1007/BF02988447

Lung uptake of intravenously injected Tc-99m-HMPAO is observed in smokers and in lung toxicity due to various agents. We investigated the Tc-99m-HMPAO uptake of bronchoalveolar lavage (BAL) cells in the lungs after incubation in in vitro conditions (6 patients), intravenous injection (IV) (7 patients) and inhalation (INH) (6 patients) of Tc-99m-HMPAO in order to show whether BAL cells are also responsible for Tc-99m-HMPAO uptake in the lungs. Cell/supernatant (C/S) count ratio was 7.0 +/- 3.5, 29.3 +/- 40.8 and 8.4 +/- 4.5 for in vitro, IV and INH groups, respectively. C/Sin vitro showed a positive correlation with % alveolar macrophages (r = 0.943, p = 0.0048) and a negative correlation with % neutrophils (r = -0.945, p = 0.0045). Cells/whole BAL fluid ratio correlated with the amount of daily cigarette consumption in INH group (r = 0.95, p = 0.0037). Tc-99m-HMPAO showed adherence to mucus after inhalation. Tc-99m-HMPAO diffuses into alveolar spaces after injection and is present in BAL fluid and BAL cells both after injection and inhalation. Glutathione concentration and oxido-reductive state of the epithelial lining fluid and BAL cells may influence the lung uptake of Tc-99m-HMPAO.