Comparison of different tissue sampling methods for protein extraction from formalin-fixed and paraffin-embedded tissue specimens

Protein extracts from formalin-fixed and paraffin-embedded (FFPE) tissue for proteomic analysis has recently gained attention. In this study, we explored the possibility to standardize tissue sampling from paraffin blocks and compared the protein extracts with those obtained from fresh frozen materi...

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Bibliographic Details
Published in:	Experimental and molecular pathology Vol. 88; no. 1; pp. 190 - 196
Main Authors:	Gräntzdörffer, Ilona, Yumlu, Saniye, Gioeva, Zarina, von Wasielewski, Reinhard, Ebert, Matthias P.A., Röcken, Christoph
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier Inc 01-02-2010 Elsevier
Subjects:	Adult Aged Autolysis - prevention & control Biological and medical sciences Blotting, Western Carcinoma, Pancreatic Ductal - chemistry Carcinoma, Pancreatic Ductal - pathology Cryopreservation Electrophoresis, Gel, Two-Dimensional Female Fixatives - chemistry Formaldehyde - chemistry Formalin-fixed and paraffin-embedded material Fresh frozen material Gastroenterology. Liver. Pancreas. Abdomen Humans Immunohistochemistry Investigative techniques, diagnostic techniques (general aspects) Liver. Biliary tract. Portal circulation. Exocrine pancreas Male Medical sciences Middle Aged Pancreatic cancer Pancreatic Neoplasms - chemistry Pancreatic Neoplasms - pathology Paraffin Embedding Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Protein extraction Proteins - analysis Proteomics Tissue Fixation - methods Tissue Preservation - methods Tissue Preservation - standards Tumors PDAC Fresh frozen material FFPE H&E Pancreatic cancer Protein extraction TMA SDS–PAGE Formalin-fixed and paraffin-embedded material PCR Materials Samplings Malignant tumor Method Paraffin Protein Tissue Anatomic pathology Pancreas cancer Digestive diseases Extraction Sampling Comparative study Cancer Pancreatic disease
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