Use of pyrosequencing to identify point mutations in domain V of 23S rRNA genes of linezolid-resistant Staphylococcus aureus and Staphylococcus epidermidis

• Published in: European journal of clinical microbiology & infectious diseases Vol. 26; no. 3; pp. 161 - 165
• Main Authors: ZHU, W, TENOVER, F. C, LIMOR, J, LONSWAY, D, PRINCE, D, DUNNE, W. M, PATEL, J. B
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01-03-2007; Springer Nature B.V
• Subjects: Acetamides - pharmacology; Anti-Infective Agents - pharmacology; Antibacterial agents; Antibiotics. Antiinfectious agents. Antiparasitic agents; Bacteria; Bacterial diseases; Base Sequence; Biological and medical sciences; DNA Primers; Drug Resistance, Bacterial; Human bacterial diseases; Humans; Infectious diseases; Linezolid; Medical sciences; Microbial Sensitivity Tests; Microbiology; Molecular Sequence Data; Mutation; Oxazolidinones - pharmacology; Pharmacology. Drug treatments; Point Mutation; Polymorphism, Single Nucleotide - genetics; RNA, Ribosomal, 23S - genetics; Staphylococcal infections, streptococcal infections, pneumococcal infections; Staphylococcus aureus; Staphylococcus aureus - drug effects; Staphylococcus aureus - genetics; Staphylococcus epidermidis; Staphylococcus epidermidis - drug effects; Staphylococcus epidermidis - genetics; Linezolid; Ribosomal DNA; Infection; Antibiotic; Staphylococcus epidermidis; Oxazolidinone derivative; Point mutation; Bacteriosis; Bacteria; Micrococcales; Micrococcaceae; Protein synthesis inhibitor; Antibacterial agent; Staphylococcal infection; Oxazole derivatives; Staphylococcus aureus
• ISSN: 0934-9723; 1435-4373
• DOI: 10.1007/s10096-007-0261-0

A pyrosequencing assay was used for the rapid characterization of linezolid-resistant isolates of Staphylococcus aureus and Staphylococcus epidermidis. The assay identified base substitutions in copies of the 23S rRNA gene and determined the percentage of alleles with the mutation. Modifications of the assay were necessary to identify all mutations in the 23S rRNA genes of S. epidermidis that were associated with linezolid resistance. A C2534T mutation was identified in S. epidermidis that was not previously reported in a linezolid-resistant isolate.