Multiple populations of neuropeptide-containing cells in cultures of the bovine adrenal medulla

Cell populations containing vasoactive intestinal polypeptide (VIP), enkephalins, and catecholamines were identified in bovine adrenal medullary cultures by immunofluorescence and radioimmunoassay. Addition of forskolin to the culture medium increased the cellular levels of both VIP and the enkephal...

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Bibliographic Details
Published in:	Brain research Vol. 349; no. 1-2; p. 267
Main Authors:	Siegel, R E, Eiden, L E, Pruss, R M
Format:	Journal Article
Language:	English
Published:	Netherlands 01-01-1985
Subjects:	Adrenal Medulla - metabolism Animals Catecholamines - metabolism Cattle Cells, Cultured Colforsin Diterpenes - pharmacology Enkephalin, Leucine - metabolism Enkephalin, Methionine - metabolism Fluorescent Antibody Technique In Vitro Techniques Nerve Tissue Proteins - metabolism Phenylethanolamine N-Methyltransferase - metabolism Radioimmunoassay Vasoactive Intestinal Peptide - metabolism
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Summary:	Cell populations containing vasoactive intestinal polypeptide (VIP), enkephalins, and catecholamines were identified in bovine adrenal medullary cultures by immunofluorescence and radioimmunoassay. Addition of forskolin to the culture medium increased the cellular levels of both VIP and the enkephalins. These changes resulted from an increase in the number of VIP-positive cells and an increase in cellular enkephalin content.
ISSN:	0006-8993
DOI:	10.1016/0165-3806(85)90152-X