Structure of the gene encoding the mouse pituitary adenylate cyclase-activating polypeptide receptor

• Published in: Gene Vol. 164; no. 2; pp. 301 - 304
• Main Authors: Aino, Hiroshi, Hashimoto, Hitoshi, Ogawa, Nobuya, Nishino, Akiko, Yamamoto, Kyohei, Nogi, Hiroyuki, Nagata, Shigekazu, Baba, Akemichi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 27-10-1995
• Subjects: 5′-flanking region; alternative splicing; amino-acid sequence; Animals; Base Composition; Base Sequence; Binding Sites; DNA - chemistry; DNA - genetics; Exons; Genes, Regulator; genomic organization; Mice - genetics; Molecular Sequence Data; Molecular Weight; PACAP; Promoter Regions, Genetic; Rats; Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide; Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I; Receptors, Pituitary Hormone - biosynthesis; Receptors, Pituitary Hormone - genetics; Restriction Mapping; Sp1 Transcription Factor - metabolism; TATA Box; VIP; aa; PACAP; alternative splicing; nt; PACAP-R; amino-acid sequence; bp; NF-I; UTR; kb; 5′-flanking region; cDNA; genomic organization; VIP; PCR
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/0378-1119(95)00391-I

The
PACAP-R gene, encoding the mouse pituitary adenylate cyclase-activating polypeptide receptor (PACAP-R), has been isolated and its structural organization has been determined.
PACAP-R spans more than 50 kb and is divided into 18 exons.
PACAP-R contains two alternative exons encoding the putative third intracellular loop, as found in the rat PACAP-R. The proximal promoter region is highly G + C rich and lacks an apparent TATA box, but contains a CCAAT box and two potential Spl-binding sites.