The MET2 gene of Saccharomyces cerevisiae: molecular cloning and nucleotide sequence

The MET2 gene of Saccharomyces cerevisiae: molecular cloning and nucleotide sequence

A 5.1-kb DNA fragment from Saccharomyces cerevisiae, which complements a yeast met2 mutant strain, has been cloned. This fragment contains the wild-type MET2 gene which codes for the homoserine O-transacetylase, one of the methionine biosynthetic enzymes. The presence of the MET2 gene has been shown...

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Bibliographic Details
Published in:Gene Vol. 49; no. 3; p. 283
Main Authors: Langin, T, Faugeron, G, Goyon, C, Nicolas, A, Rossignol, J L
Format: Journal Article
Language:English
Published: Netherlands 1986
Subjects:
Acetyltransferases - genetics
Amino Acid Sequence
Base Sequence
Cloning, Molecular
Codon
Genes
Genes, Fungal
Methionine - genetics
RNA, Messenger - genetics
Saccharomyces cerevisiae - genetics
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Summary:A 5.1-kb DNA fragment from Saccharomyces cerevisiae, which complements a yeast met2 mutant strain, has been cloned. This fragment contains the wild-type MET2 gene which codes for the homoserine O-transacetylase, one of the methionine biosynthetic enzymes. The presence of the MET2 gene has been shown by integrative transformation experiments and genetic analyses of the resulting transformants. The complete nucleotide sequence of a 2826-bp DNA fragment carrying the MET2 gene has been determined. The sequence contains one major open reading frame of 438 codons, giving a calculated Mr of 48,370 for the encoded protein. We have identified the transcriptional product of the MET2 gene and estimated its size at 1650 nucleotides.
ISSN:0378-1119
DOI:10.1016/0378-1119(86)90364-1